ArticleACS synthetic biology2014
Biological 2-input decoder circuit in human cells.
Article in ACS synthetic biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Perfluorooctanoic Acid and Its Short-Chain Substitutes Induce Cytotoxic and Prooxidative Changes in Human Peripheral Blood Mononuclear Cells: A Comparative Study.International journal of molecular sciences · 2025Article
- Cell morphology-based machine learning models for human cell state classification.NPJ systems biology and applications · 2021Article
- Towards control of cellular decision-making networks in the epithelial-to-mesenchymal transition.Physical biology · 2019Review
- Mapping the operational landscape of microRNAs in synthetic gene circuits.NPJ systems biology and applications · 2018Article
- Large-scale design of robust genetic circuits with multiple inputs and outputs for mammalian cells.Nature biotechnology · 2017Article
- Reconfigurable hybrid interface for molecular marker diagnostics and in-situ reporting.Biosensors & bioelectronics · 2015Article
- Latent phenotypes pervade gene regulatory circuits.BMC systems biology · 2014Article
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Authors and funding
2 authors.
Funding
Abstract
Decoders are combinational circuits that convert information from n inputs to a maximum of 2(n) outputs. This operation is of major importance in computing systems yet it is vastly underexplored in synthetic biology. Here, we present a synthetic gene network architecture that operates as a biological decoder in human cells, converting 2 inputs to 4 outputs. As a proof-of-principle, we use small molecules to emulate the two inputs and fluorescent reporters as the corresponding four outputs. The experiments are performed using transient transfections in human kidney embryonic cells and the characterization by fluorescence microscopy and flow cytometry. We show a clear separation between the ON and OFF mean fluorescent intensity states. Additionally, we adopt the integrated mean fluorescence intensity for the characterization of the circuit and show that this metric is more robust to transfection conditions when compared to the mean fluorescent intensity. To conclude, we present the first implementation of a genetic decoder. This combinational system can be valuable toward engineering higher-order circuits as well as accommodate a multiplexed interface with endogenous cellular functions.
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