Evidence map›Paper›PMID 24693195›Full record

ArticleJournal of stem cells & regenerative medicine2012

Allogeneic amniotic membrane-derived mesenchymal stromal cell transplantation in a porcine model of chronic myocardial ischemia.

M Kimura, M Toyoda, S Gojo, Y Itakura, D Kami, S Miyoshi, S Kyo, M Ono, A Umezawa

Open access · goldAbstract read
PubMed Full text at PMC
In one paragraph

Article in Journal of stem cells & regenerative medicine, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
0.5field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 16 citations in OpenAlex.

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  11. Research Advancements in Porcine Derived Mesenchymal Stem Cells.Current stem cell research & therapy · 2016
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

M KimuraDepartment of Cardiovascular Surgery, Graduate School of Medicine, The University of Tokyo , Tokyo, Japan ; Equally contributed to the study.
M ToyodaResearch Team for Geriatric Medicine, Tokyo Metropolitan Institute of Gerontology , Tokyo, Japan ; Equally contributed to the study.
S GojoDepartment of Cardiovascular Medicine, Kyoto Prefectural University of Medicine , Kyoto, Japan.
Y ItakuraResearch Team for Geriatric Medicine, Tokyo Metropolitan Institute of Gerontology , Tokyo, Japan.
D KamiDepartment of Cardiovascular Medicine, Kyoto Prefectural University of Medicine , Kyoto, Japan.
S MiyoshiDepartment of Cardiology, Keio University School of Medicine , Tokyo, Japan.
S KyoDepartment of Therapeutic Strategy for Heart Failure, Graduate School of Medicine, The University of Tokyo , Tokyo, Japan.
M OnoDepartment of Cardiovascular Surgery, Graduate School of Medicine, The University of Tokyo , Tokyo, Japan.
A UmezawaDepartment of Reproductive Biology, National Research Institute for Child Health and Development , Tokyo, Japan.
Keio University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction. Amniotic membrane contains a multipotential stem cell population and is expected to possess the machinery to regulate immunological reactions. We investigated the safety and efficacy of allogeneic amniotic membrane-derived mesenchymal stromal cell (AMSC) transplantation in a porcine model of chronic myocardial ischemia as a preclinical trial. Methods. Porcine AMSCs were isolated from amniotic membranes obtained by cesarean section just before delivery and were cultured to increase their numbers before transplantation. Chronic myocardial ischemia was induced by implantation of an ameroid constrictor around the left circumflex coronary artery. Four weeks after ischemia induction, nine swine were assigned to undergo either allogeneic AMSC transplantation or normal saline injection. Functional analysis was performed by echocardiography, and histological examinations were carried out by immunohistochemistry 4 weeks after AMSC transplantation. Results. Echocardiography demonstrated that left ventricular ejection fraction was significantly improved and left ventricular dilatation was well attenuated 4 weeks after AMSC transplantation. Histological assessment showed a significant reduction in percentage of fibrosis in the AMSC transplantation group. Injected allogeneic green fluorescent protein (GFP)-expressing AMSCs were identified in the immunocompetent host heart without the use of any immunosuppressants 4 weeks after transplantation. Immunohistochemistry revealed that GFP colocalized with cardiac troponin T and cardiac troponin I. Conclusions. We have demonstrated that allogeneic AMSC transplantation produced histological and functional improvement in the impaired myocardium in a porcine model of chronic myocardial ischemia. The transplanted allogeneic AMSCs survived without the use of any immunosuppressants and gained cardiac phenotype through either their transdifferentiation or cell fusion.

Indexed as

Allogeneic cell transplantationAmniotic membraneChronic myocardial ischemiaMesenchymal stromal cellPorcine model

Identifiers

PMID24693195
PMCPMC3908291
OpenAlexW2142195306

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.