ArticleJournal of experimental botany2014
Isopentenyltransferase-1 (IPT1) knockout in Physcomitrella together with phylogenetic analyses of IPTs provide insights into evolution of plant cytokinin biosynthesis.
Article in Journal of experimental botany, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 69 citations in OpenAlex.
- KAI2-dependent signaling controls vegetative reproduction in Marchantia polymorpha through activation of LOG-mediated cytokinin synthesis (14).Nature communications · 2025Article
- Overexpression of MtIPT gene enhanced drought tolerance and delayed leaf senescence of creeping bentgrass (Agrostis stolonifera L.).BMC plant biology · 2024Article
- Cytokinin: From autoclaved DNA to two-component signaling.The Plant cell · 2024Review
- Regulation of developmental gatekeeping and cell fate transition by the calpain protease DEK1 in Physcomitrium patens.Communications biology · 2024Article
- The molecular mechanism by which heat stress during the grain filling period inhibits maize grain filling and reduces yield.Frontiers in plant science · 2024Article
- The origin and early evolution of cytokinin signaling.Frontiers in plant science · 2023Review
- Red Light and Glucose Enhance Cytokinin-Mediated Bud Initial Formation inPlants (Basel, Switzerland) · 2022Article
- Genetic Divergence of Lineage-Specific Tandemly Duplicated Gene Clusters in Four Diploid Potato Genotypes.Frontiers in plant science · 2022Article
- Cytokinin Perception in Ancient Plants beyond Angiospermae.International journal of molecular sciences · 2021Article
- Review
- Isopentenyltransferases as master regulators of crop performance: their function, manipulation, and genetic potential for stress adaptation and yield improvement.Plant biotechnology journal · 2021Review
- Cytokinin oxidase PpCKX1 plays regulatory roles in development and enhances dehydration and salt tolerance in Physcomitrella patens.Plant cell reports · 2020Article
- Cytokinins Are Abundant and Widespread Among Insect Species.Plants (Basel, Switzerland) · 2020Article
- Evolution and roles of cytokinin genes in angiosperms 1: Do ancientHorticulture research · 2020Article
- Evolution and roles of cytokinin genes in angiosperms 2: Do ancientHorticulture research · 2020Article
- PhytopathogenicFrontiers in microbiology · 2020Article
- Cytokinins in Dictyostelia - A Unique Model for Studying the Functions of Signaling Agents From Species to Kingdoms.Frontiers in cell and developmental biology · 2020Review
- The Origins and Roles of Methylthiolated Cytokinins: Evidence From Among Life Kingdoms.Frontiers in cell and developmental biology · 2020Review
- Cytokinin Detection during theBiomolecules · 2019Article
- The initial deficiency of protein processing and flavonoids biosynthesis were the main mechanisms for the male sterility induced by SX-1 in Brassica napus.BMC genomics · 2018Article
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The moss Physcomitrella patens is part of an early divergent clade of land plants utilizing the plant hormone cytokinin for growth control. The rate-limiting step of cytokinin biosynthesis is mediated by isopentenyltransferases (IPTs), found in land plants either as adenylate-IPTs or as tRNA-IPTs. Although a dominant part of cytokinins in flowering plants are synthesized by adenylate-IPTs, the Physcomitrella genome only encodes homologues of tRNA-IPTs. This study therefore looked into the question of whether cytokinins in moss derive from tRNA exclusively. Targeted gene knockout of ipt1 (d|ipt1) along with localization studies revealed that the chloroplast-bound IPT1 was almost exclusively responsible for the A37 prenylation of tRNA in Physcomitrella. Ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)-based cytokinin profiling demonstrated that the total amount of all free cytokinins in tissue was almost unaffected. However, the knockout plants showed increased levels of the N (6) -isopentenyladenine (iP)- and trans-zeatin (tZ)-type cytokinins, considered to provide active forms, while cis-zeatin (cZ)-type cytokinins were reduced. The data provide evidence for an additional and unexpected tRNA-independent cytokinin biosynthetic pathway in moss. Comprehensive phylogenetic analysis indicates a diversification of tRNA-IPT-like genes in bryophytes probably related to additional functions.
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