ReviewScience signaling2014
Visualizing and manipulating temporal signaling dynamics with fluorescence-based tools.
Review in Science signaling, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed.
- sUPRa is a dual-color reporter for unbiased quantification of the unfolded protein response with cellular resolution.Scientific reports · 2024Article
- The Provenance, Providence, and Position of Endothelial Cells in Injured Spinal Cord Vascular Pathology.Cellular and molecular neurobiology · 2023Review
- Signalling dynamics, cell decisions, and homeostatic control in health and disease.Current opinion in cell biology · 2022Review
- Subcellular Dynamic Immunopatterning of Cytosolic Protein Complexes on Microstructured Polymer Substrates.ACS sensors · 2021Article
- Ascorbic Acid Supplementation Improves Skeletal Muscle Growth in Pacu (International journal of molecular sciences · 2021Article
- Cell Tracking for Organoids: Lessons From Developmental Biology.Frontiers in cell and developmental biology · 2021Review
- Efficient photoactivatable Dre recombinase for cell type-specific spatiotemporal control of genome engineering in the mouse.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Imaging dynamic cell signaling in vivo with new classes of fluorescent reporters.Current opinion in chemical biology · 2020Review
- In vivo study of gene expression with an enhanced dual-color fluorescent transcriptional timer.eLife · 2019Article
- Synergistic Ensemble of Optogenetic Actuators and Dynamic Indicators in Cell Biology.Molecules and cells · 2018Review
- Visualizing Dynamics of Cell Signaling In Vivo with a Phase Separation-Based Kinase Reporter.Molecular cell · 2018Article
- System identification of signaling dependent gene expression with different time-scale data.PLoS computational biology · 2017Article
- Coupling optogenetics and light-sheet microscopy, a method to study Wnt signaling during embryogenesis.Scientific reports · 2017Article
- The temporal basis of angiogenesis.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Review
- Molecular mechanisms underlying alcohol-drinking behaviours.Nature reviews. Neuroscience · 2016Review
- Mapping signaling pathway cross-talk in Drosophila cells.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- High-Content Quantification of Single-Cell Immune Dynamics.Cell reports · 2016Article
- Spatial and temporal organization of signaling pathways.Trends in biochemical sciences · 2014Review
- Reversible protein inactivation by optogenetic trapping in cells.Nature methods · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
The use of genome-wide proteomic and RNA interference approaches has moved our understanding of signal transduction from linear pathways to highly integrated networks centered on core nodes. However, probing the dynamics of flow of information through such networks remains technically challenging. In particular, how the temporal dynamics of an individual pathway can elicit distinct outcomes in a single cell type and how multiple pathways may interact sequentially or synchronously to influence cell fate remain open questions in many contexts. The development of fluorescence-based reporters and optogenetic regulators of pathway activity enables the analysis of signaling in living cells and organisms with unprecedented spatiotemporal resolution and holds the promise of addressing these key questions. We present a brief overview of the evidence for the importance of temporal dynamics in cellular regulation, introduce these fluorescence-based tools, and highlight specific studies that leveraged these tools to probe the dynamics of information flow through signaling networks. In particular, we highlight two studies in Caenorhabditis elegans sensory neurons and cultured mammalian cells that demonstrate the importance of signal dynamics in determining cellular responses.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.