ArticleChembiochem : a European journal of chemical biology2014
Using singular value decomposition to characterize protein-protein interactions by in-cell NMR spectroscopy.
Article in Chembiochem : a European journal of chemical biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Radio Signals from Live Cells: The Coming of Age of In-Cell Solution NMR.Chemical reviews · 2022Review
- Active metabolism unmasks functional protein-protein interactions in real time in-cell NMR.Communications biology · 2020Article
- First hyperpolarized [2-Journal of magnetic resonance (San Diego, Calif. : 1997) · 2019Article
- Controlling and quantifying protein concentration in Escherichia coli.Protein science : a publication of the Protein Society · 2019Article
- The Inescapable Effects of Ribosomes on In-Cell NMR Spectroscopy and the Implications for Regulation of Biological Activity.International journal of molecular sciences · 2019Review
- In-Cell NMR Spectroscopy of Intrinsically Disordered Proteins.Proteomics · 2019Review
- Interaction proteomics by using in-cell NMR spectroscopy.Journal of proteomics · 2019Review
- Applications of In-Cell NMR in Structural Biology and Drug Discovery.International journal of molecular sciences · 2019Review
- Improved sensitivity and resolution of in-cell NMR spectra.Methods in enzymology · 2019Article
- Potent Inhibitors of Mycobacterium tuberculosis Growth Identified by Using in-Cell NMR-based Screening.ACS chemical biology · 2018Article
- Real-Time In-Cell Nuclear Magnetic Resonance: Ribosome-Targeted Antibiotics Modulate Quinary Protein Interactions.Biochemistry · 2018Article
- Quantitative Determination of Interacting Protein Surfaces in Prokaryotes and Eukaryotes by Using In-Cell NMR Spectroscopy.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Tracking Equilibrium and Nonequilibrium Shifts in Data with TREND.Biophysical journal · 2017Article
- Binding Isotherms and Time Courses Readily from Magnetic Resonance.Analytical chemistry · 2016Article
- Caught in action: selecting peptide aptamers against intrinsically disordered proteins in live cells.Scientific reports · 2015Article
- A tool set to map allosteric networks through the NMR chemical shift covariance analysis.Scientific reports · 2014Article
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4 authors.
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Abstract
Distinct differences between how model proteins interact in-cell and in vitro suggest that the cytosol might have a profound effect in modulating protein-protein and/or protein-ligand interactions that are not observed in vitro. Analyses of in-cell NMR spectra of target proteins interacting with physiological partners are further complicated by low signal-to-noise ratios, and the long overexpression times used in protein-protein interaction studies may lead to changes in the in-cell spectra over the course of the experiment. To unambiguously resolve the principal binding mode between two interacting species against the dynamic cellular background, we analyzed in-cell spectral data of a target protein over the time course of overexpression of its interacting partner by using single-value decomposition (SVD). SVD differentiates between concentration-dependent and concentration-independent events and identifies the principal binding mode between the two species. The analysis implicates a set of amino acids involved in the specific interaction that differs from previous NMR analyses but is in good agreement with crystallographic data.
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