ArticlePloS one2014
From single cells to tissues: interactions between the matrix and human breast cells in real time.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 49 citations in OpenAlex.
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- Modeling Mammary Organogenesis from Biological First Principles: A Systems Biology Approach.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Cell Mechanics Regulates the Dynamic Anisotropic Remodeling of Fibril Matrix at Large Scale.Research (Washington, D.C.) · 2023Article
- TiAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Matrix Composition Modulates Vitamin D3's Effects on 3D Collagen Fiber Organization by MCF10A Cells.Tissue engineering. Part A · 2021Article
- BAD regulates mammary gland morphogenesis by 4E-BP1-mediated control of localized translation in mouse and human models.Nature communications · 2021Article
- Tissue Imaging and Quantification Relying on Endogenous Contrast.Advances in experimental medicine and biology · 2021Review
- Environmental exposures, stem cells, and cancer.Pharmacology & therapeutics · 2019Review
- Vitamin D3 constrains estrogen's effects and influences mammary epithelial organization in 3D cultures.Scientific reports · 2019Article
- 3D organizational mapping of collagen fibers elucidates matrix remodeling in a hormone-sensitive 3D breast tissue model.Biomaterials · 2018Article
- Ultrasound shear wave elastography of breast lesions: correlation of anisotropy with clinical and histopathological findings.Cancer imaging : the official publication of the International Cancer Imaging Society · 2018Article
- Characterization of MCF-12A cell phenotype, response to estrogens, and growth in 3D.Cancer cell international · 2018Article
- Automated quantification of three-dimensional organization of fiber-like structures in biological tissues.Biomaterials · 2017Article
- Modeling mammary organogenesis from biological first principles: Cells and their physical constraints.Progress in biophysics and molecular biology · 2016Review
- Biomaterials-based strategies for salivary gland tissue regeneration.Biomaterials science · 2016Article
- A Hormone-responsive 3D Culture Model of the Human Mammary Gland Epithelium.Journal of visualized experiments : JoVE · 2016Article
- Patterning of Fibroblast and Matrix Anisotropy within 3D Confinement is Driven by the Cytoskeleton.Advanced healthcare materials · 2016Article
- Environmental endocrine disruptors: Effects on the human male reproductive system.Reviews in endocrine & metabolic disorders · 2015Review
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 3 countries.
Funding
Abstract
backgroundMammary gland morphogenesis involves ductal elongation, branching, and budding. All of these processes are mediated by stroma--epithelium interactions. Biomechanical factors, such as matrix stiffness, have been established as important factors in these interactions. For example, epithelial cells fail to form normal acinar structures in vitro in 3D gels that exceed the stiffness of a normal mammary gland. Additionally, heterogeneity in the spatial distribution of acini and ducts within individual collagen gels suggests that local organization of the matrix may guide morphogenesis. Here, we quantified the effects of both bulk material stiffness and local collagen fiber arrangement on epithelial morphogenesis.
resultsThe formation of ducts and acini from single cells and the reorganization of the collagen fiber network were quantified using time-lapse confocal microscopy. MCF10A cells organized the surrounding collagen fibers during the first twelve hours after seeding. Collagen fiber density and alignment relative to the epithelial surface significantly increased within the first twelve hours and were a major influence in the shaping of the mammary epithelium. The addition of Matrigel to the collagen fiber network impaired cell-mediated reorganization of the matrix and increased the probability of spheroidal acini rather than branching ducts. The mechanical anisotropy created by regions of highly aligned collagen fibers facilitated elongation and branching, which was significantly correlated with fiber organization. In contrast, changes in bulk stiffness were not a strong predictor of this epithelial morphology.
conclusionsLocalized regions of collagen fiber alignment are required for ductal elongation and branching suggesting the importance of local mechanical anisotropy in mammary epithelial morphogenesis. Similar principles may govern the morphology of branching and budding in other tissues and organs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.