ArticleThe Review of scientific instruments2014
Design and evaluation of a device for fast multispectral time-resolved fluorescence spectroscopy and imaging.
Article in The Review of scientific instruments, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed.
- Intraoperative Mapping of Parathyroid Glands Using Fluorescence Lifetime Imaging.The Journal of surgical research · 2021Trial
- Effects of surgical resection on tissue autofluorescence lifetime signatures in head and neck cancer: implications for intraoperative tumor margin assessment.Journal of biomedical optics · 2026Article
- Label-free fluorescence lifetime imaging for rapid discrimination of high-grade prostate cancer in fresh biopsy cores: a feasibility study.Journal of biomedical optics · 2026Article
- Investigating probe-receptor interactions and enhancing fluorescence guided surgery with fluorescence lifetime imaging and NF-800 in HER-2 positive esophageal adenocarcinoma.Scientific reports · 2025Article
- Discriminating single-molecule binding events from diffraction-limited fluorescence.Nature communications · 2025Article
- Data-Centric Learning Framework for Real-Time Detection of Aiming Beam in Fluorescence Lifetime Imaging Guided Surgery.IEEE transactions on bio-medical engineering · 2025Article
- Metabolic light absorption, scattering, and emission (MetaLASE) microscopy.Science advances · 2024Article
- Immunoassay System Based on the Technology of Time-Resolved Fluorescence Resonance Energy Transfer.Sensors (Basel, Switzerland) · 2024Article
- Applications of machine learning in time-domain fluorescence lifetime imaging: a review.Methods and applications in fluorescence · 2024Review
- Fibre-optic based exploration of lung cancer autofluorescence using spectral fluorescence lifetime.Biomedical optics express · 2024Article
- Anatomy-Specific Classification Model Using Label-Free FLIm to Aid Intraoperative Surgical Guidance of Head and Neck Cancer.IEEE transactions on bio-medical engineering · 2023Article
- Intraoperative molecular imaging: 3rd biennial clinical trials update.Journal of biomedical optics · 2023Review
- In vivo characterization of the human glioblastoma infiltrative edge with label-free intraoperative fluorescence lifetime imaging.Biomedical optics express · 2023Article
- Intraoperative detection of IDH-mutant glioma using fluorescence lifetime imaging.Journal of biophotonics · 2023Article
- Multimodal fluorescence lifetime imaging and optical coherence tomography for longitudinal monitoring of tissue-engineered cartilage maturation in a preclinical implantation model.Journal of biomedical optics · 2023Article
- Intraoperative delineation of p16+ oropharyngeal carcinoma of unknown primary origin with fluorescence lifetime imaging: Preliminary report.Head & neck · 2022Article
- Assessment of Murine Colon Inflammation Using Intraluminal Fluorescence Lifetime Imaging.Molecules (Basel, Switzerland) · 2022Article
- Single Photon Avalanche Diode Arrays for Time-Resolved Raman Spectroscopy.Sensors (Basel, Switzerland) · 2021Review
- Multispectral fluorescence lifetime imaging device with a silicon avalanche photodetector.Optics express · 2021Article
- Mesoscopic fluorescence lifetime imaging: Fundamental principles, clinical applications and future directions.Journal of biophotonics · 2021Review
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
The application of time-resolved fluorescence spectroscopy (TRFS) to in vivo tissue diagnosis requires a method for fast acquisition of fluorescence decay profiles in multiple spectral bands. This study focusses on development of a clinically compatible fiber-optic based multispectral TRFS (ms-TRFS) system together with validation of its accuracy and precision for fluorescence lifetime measurements. It also presents the expansion of this technique into an imaging spectroscopy method. A tandem array of dichroic beamsplitters and filters was used to record TRFS decay profiles at four distinct spectral bands where biological tissue typically presents fluorescence emission maxima, namely, 390, 452, 542, and 629 nm. Each emission channel was temporally separated by using transmission delays through 200 μm diameter multimode optical fibers of 1, 10, 19, and 28 m lengths. A Laguerre-expansion deconvolution algorithm was used to compensate for modal dispersion inherent to large diameter optical fibers and the finite bandwidth of detectors and digitizers. The system was found to be highly efficient and fast requiring a few nano-Joule of laser pulse energy and <1 ms per point measurement, respectively, for the detection of tissue autofluorescent components. Organic and biological chromophores with lifetimes that spanned a 0.8-7 ns range were used for system validation, and the measured lifetimes from the organic fluorophores deviated by less than 10% from values reported in the literature. Multi-spectral lifetime images of organic dye solutions contained in glass capillary tubes were recorded by raster scanning the single fiber probe in a 2D plane to validate the system as an imaging tool. The lifetime measurement variability was measured indicating that the system provides reproducible results with a standard deviation smaller than 50 ps. The ms-TRFS is a compact apparatus that makes possible the fast, accurate, and precise multispectral time-resolved fluorescence lifetime measurements of low quantum efficiency sub-nanosecond fluorophores.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.