ArticlePeerJ2014
Regulation of antimycin biosynthesis by the orphan ECF RNA polymerase sigma factor σ (AntA.).
Article in PeerJ, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 36 citations in OpenAlex.
- Complete Genome Sequencing of Streptomyces sp. Qhu-M197 Isolated from Alpine Meadows in the Qinghai-Tibetan Plateau Uncovers Biosynthetic Gene Clusters Encoding Bioactive Natural Products.Biochemical genetics · 2026Article
- Regulatory and metabolic control of microbial biosynthetic gene clusters.Communications biology · 2026Review
- Scalable secondary metabolite production in Streptomyces using a plug-and-play system.Nature biotechnology · 2026Article
- Discovery of thiazostatin D/E using UPLC-HR-MS2-based metabolomics and σ-factor engineering ofFrontiers in bioengineering and biotechnology · 2024Article
- Mechanisms of Action of Non-Canonical ECF Sigma Factors.International journal of molecular sciences · 2022Review
- Competition-based screening helps to secure the evolutionary stability of a defensive microbiome.BMC biology · 2021Article
- Expansion and re-classification of the extracytoplasmic function (ECF) σ factor family.Nucleic acids research · 2021Article
- Article
- A chromatogram-simplified Streptomyces albus host for heterologous production of natural products.Antonie van Leeuwenhoek · 2020Article
- Dissolution of the Disparate: Co-ordinate Regulation in Antibiotic Biosynthesis.Antibiotics (Basel, Switzerland) · 2019Review
- Regulation of specialised metabolites in Actinobacteria - expanding the paradigms.Environmental microbiology reports · 2018Review
- A phylogenetic and evolutionary analysis of antimycin biosynthesis.Microbiology (Reading, England) · 2018Article
- Connecting Metabolic Pathways: Sigma Factors inFrontiers in microbiology · 2017Review
- The Draft Genome Sequence of Actinokineospora bangkokensis 44EHWMolecules (Basel, Switzerland) · 2016Article
- Engineering microbial hosts for production of bacterial natural products.Natural product reports · 2016Review
- Genetic manipulation of secondary metabolite biosynthesis for improved production in Streptomyces and other actinomycetes.Journal of industrial microbiology & biotechnology · 2016Review
- Article
- Metabolomic profiling and genomic study of a marine sponge-associated Streptomyces sp.Marine drugs · 2014Article
- Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Antimycins are an extended family of depsipeptides that are made by filamentous actinomycete bacteria and were first isolated more than 60 years ago. Recently, antimycins have attracted renewed interest because of their activities against the anti-apoptotic machineries inside human cells which could make them promising anti-cancer compounds. The biosynthetic pathway for antimycins was recently characterised but very little is known about the organisation and regulation of the antimycin (ant) gene cluster. Here we report that the ant gene cluster in Streptomyces albus is organized into four transcriptional units; the antBA, antCDE, antGF and antHIJKLMNO operons. Unusually for secondary metabolite clusters, the antG and antH promoters are regulated by an extracytoplasmic function (ECF) RNA polymerase sigma factor named σ (AntA) which represents a new sub-family of ECF σ factors that is only found in antimycin producing strains. We show that σ (AntA) controls production of the unusual precursor 3-aminosalicylate which is absolutely required for the production of antimycins. σ (AntA) is highly conserved in antimycin producing strains and the -10 and -35 elements at the σ (AntA) regulated antG and antH promoters are also highly conserved suggesting a common mechanism of regulation. We also demonstrate that altering the C-terminal Ala-Ala residues found in all σ (AntA) proteins to Asp-Asp increases expression of the antFG and antGHIJKLMNO operons and we speculate that this Ala-Ala motif may be a signal for the protease ClpXP.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.