ArticleBiomedical optics express2014
Spatial light modulator based active wide-field illumination for ex vivo and in vivo quantitative NIR FRET imaging.
Article in Biomedical optics express, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 39 citations in OpenAlex.
- Design and characterization of an optical phantom for mesoscopic multimodal fluorescence lifetime imaging and optical coherence elastography.Biomedical optics express · 2025Article
- Fluorescence Lifetime Imaging for Quantification of Targeted Drug Delivery in Varying Tumor Microenvironments.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Dynamic control and manipulation of near-fields using direct feedback.Light, science & applications · 2024Article
- Fluorescence Lifetime Imaging for Quantification of Targeted Drug Delivery in Varying Tumor Microenvironments.bioRxiv : the preprint server for biology · 2024Article
- In vivo quantitative FRET small animal imaging: Intensity versus lifetime-based FRET.Biophysical reports · 2023Article
- Article
- Macroscopic Fluorescence Lifetime Imaging for Monitoring of Drug-Target Engagement.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Quantification of Trastuzumab-HER2 Engagement In Vitro and In Vivo.Molecules (Basel, Switzerland) · 2020Article
- Multiplexed non-invasive tumor imaging of glucose metabolism and receptor-ligand engagement using dark quencher FRET acceptor.Theranostics · 2020Article
- Fast fit-free analysis of fluorescence lifetime imaging via deep learning.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- In vitro and in vivo phasor analysis of stoichiometry and pharmacokinetics using short-lifetime near-infrared dyes and time-gated imaging.Journal of biophotonics · 2019Article
- Comparison of illumination geometry for lifetime-based measurements in whole-body preclinical imaging.Journal of biophotonics · 2018Article
- Quantitative imaging of receptor-ligand engagement in intact live animals.Journal of controlled release : official journal of the Controlled Release Society · 2018Article
- Review of structured light in diffuse optical imaging.Journal of biomedical optics · 2018Review
- Compressive hyperspectral time-resolved wide-field fluorescence lifetime imaging.Nature photonics · 2017Article
- Comparison of tomographic fluorescence spectral and lifetime multiplexing.Optics letters · 2016Article
- Generalized mesh-based Monte Carlo for wide-field illumination and detection via mesh retessellation.Biomedical optics express · 2016Article
- Assessment of Gate Width Size on Lifetime-Based Förster Resonance Energy Transfer Parameter Estimation.Photonics · 2015Article
- Hyperspectral time-resolved wide-field fluorescence molecular tomography based on structured light and single-pixel detection.Optics letters · 2015Article
- Temporal Data Set Reduction Based on D-Optimality for Quantitative FLIM-FRET Imaging.PloS one · 2015Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Fluorescence lifetime imaging is playing an increasing role in drug development by providing a sensitive method to monitor drug delivery and receptor-ligand interactions. However, the wide dynamic range of fluorescence intensity emitted by ex vivo and in vivo samples presents challenges in retrieving information over the whole subject accurately and quantitatively. To overcome this challenge, we developed an active wide-field illumination (AWFI) strategy based on a spatial light modulator that acquires optimal fluorescence signals by enhancing the dynamic range, signal to noise ratio, and estimation of lifetime-based parameters. We demonstrate the ability of AWFI to estimate Förster resonance energy transfer (FRET) donor fraction from dissected organs with high accuracy (standard deviation <6%) over the whole field of view, in contrast with the homogenous wide-field illumination. We further report its successful application to quantitative FRET imaging in a live mouse. AWFI allows improved detection of weak signals and enhanced quantitative accuracy in ex vivo and in vivo molecular fluorescence quantitative imaging. The technique allows for robust quantitative estimation of the bio-distribution of molecular probes and lifetime-based parameters over an extended imaging field exhibiting a large range of fluorescence intensities and at a high acquisition speed (less than 1 min).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.