ArticleBiomedical optics express2014
TestSTORM: Simulator for optimizing sample labeling and image acquisition in localization based super-resolution microscopy.
Article in Biomedical optics express, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Revisiting PSF models: Unifying framework and high-performance implementation.Journal of microscopy · 2026Article
- ProDOL: a general method to determine the degree of labeling for staining optimization and molecular counting.Nature methods · 2024Article
- Triggered cagedSTORM microscopy.Biomedical optics express · 2024Article
- DETECTOR: structural information guided artifact detection for super-resolution fluorescence microscopy image.Biomedical optics express · 2021Article
- MTrack: Automated Detection, Tracking, and Analysis of Dynamic Microtubules.Scientific reports · 2019Article
- Switchable Fluorophores for Single-Molecule Localization Microscopy.Chemical reviews · 2018Review
- Super-resolution imaging of subcortical white matter using stochastic optical reconstruction microscopy (STORM) and super-resolution optical fluctuation imaging (SOFI).Neuropathology and applied neurobiology · 2018Article
- Quality of biological images, reconstructed using localization microscopy data.Bioinformatics (Oxford, England) · 2018Article
- TestSTORM: Versatile simulator software for multimodal super-resolution localization fluorescence microscopy.Scientific reports · 2017Article
- Local dimensionality determines imaging speed in localization microscopy.Nature communications · 2017Article
- Navigating challenges in the application of superresolution microscopy.The Journal of cell biology · 2017Review
- Simulated single molecule microscopy with SMeagol.Bioinformatics (Oxford, England) · 2016Article
- From single-molecule spectroscopy to super-resolution imaging of the neuron: a review.Methods and applications in fluorescence · 2016Review
- Important factors determining the nanoscale tracking precision of dynamic microtubule ends.Journal of microscopy · 2016Article
- Ellipsoid Localization Microscopy Infers the Size and Order of Protein Layers in Bacillus Spore Coats.Biophysical journal · 2015Article
- Resolution improvement by 3D particle averaging in localization microscopy.Methods and applications in fluorescence · 2015Article
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Authors and funding
8 authors.
Funding
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Abstract
Localization-based super-resolution microscopy image quality depends on several factors such as dye choice and labeling strategy, microscope quality and user-defined parameters such as frame rate and number as well as the image processing algorithm. Experimental optimization of these parameters can be time-consuming and expensive so we present TestSTORM, a simulator that can be used to optimize these steps. TestSTORM users can select from among four different structures with specific patterns, dye and acquisition parameters. Example results are shown and the results of the vesicle pattern are compared with experimental data. Moreover, image stacks can be generated for further evaluation using localization algorithms, offering a tool for further software developments.
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