ArticleBiomedical optics express2014
4D tracking of clinical seminal samples for quantitative characterization of motility parameters.
Article in Biomedical optics express, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Label-free, high-throughput holographic imaging to evaluate mammalian gametes and embryos†.Biology of reproduction · 2024Review
- Multi-Illumination Single-Holographic-Exposure Lensless Fresnel (MISHELF) Microscopy: Principles and Biomedical Applications.Sensors (Basel, Switzerland) · 2023Review
- Disposable paper-based microfluidics for fertility testing.iScience · 2022Review
- HoloPhaseNet: fully automated deep-learning-based hologram reconstruction using a conditional generative adversarial model.Biomedical optics express · 2022Article
- CatSper and its CaM-like CaFASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2022Article
- Human live spermatozoa morphology assessment using digital holographic microscopy.Scientific reports · 2022Article
- Design, Calibration, and Application of a Robust, Cost-Effective, and High-Resolution Lensless Holographic Microscope.Sensors (Basel, Switzerland) · 2022Article
- Advances in sperm analysis: techniques, discoveries and applications.Nature reviews. Urology · 2021Review
- Machine learning for sperm selection.Nature reviews. Urology · 2021Review
- Limited-angle tomographic phase microscopy utilizing confocal scanning fluorescence microscopy.Biomedical optics express · 2021Article
- Reproductive outcomes predicted by phase imaging with computational specificity of spermatozoon ultrastructure.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- High-resolution 4-D acquisition of freely swimming human sperm cells without staining.Science advances · 2020Article
- The Use of Motion Analysis as Particle Biomarkers in Lensless Optofluidic Projection Imaging for Point of Care Urine Analysis.Scientific reports · 2019Article
- Combined Raman and polarization sensitive holographic imaging for a multimodal label-free assessment of human sperm function.Scientific reports · 2019Article
- 3D imaging of sex-sorted bovine spermatozoon locomotion, head spin and flagellum beating.Scientific reports · 2018Article
- Sub-Diffraction Visible Imaging Using Macroscopic Fourier Ptychography and Regularization by Denoising.Sensors (Basel, Switzerland) · 2018Article
- Label-free 3D computational imaging of spermatozoon locomotion, head spin and flagellum beating over a large volume.Light, science & applications · 2018Article
- Simultaneous two-color imaging in digital holographic microscopy.Optics express · 2017Article
- Endowing a plain fluidic chip with micro-optics: a holographic microscope slide.Light, science & applications · 2017Article
- Computational imaging of sperm locomotion.Biology of reproduction · 2017Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this paper we investigate the use of a digital holographic microscope, with partial spatial coherent illumination, for the automated detection and tracking of spermatozoa. This in vitro technique for the analysis of quantitative parameters is useful for assessment of semen quality. In fact, thanks to the capabilities of digital holography, the developed algorithm allows us to resolve in-focus amplitude and phase maps of the cells under study, independently of focal plane of the sample image. We have characterized cell motility on clinical samples of seminal fluid. In particular, anomalous sperm cells were characterized and the quantitative motility parameters were compared to those of normal sperm.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.