ReviewComputational and structural biotechnology journal2013
Targeted, LCMS-based Metabolomics for Quantitative Measurement of NAD(+) Metabolites.
Review in Computational and structural biotechnology journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 117 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
117 citing papers in PubMed, 197 citations in OpenAlex.
- A randomized placebo-controlled trial of nicotinamide riboside in older adults with mild cognitive impairment.GeroScience · 2024Trial
- Nicotinamide Riboside Augments the Aged Human Skeletal Muscle NADCell reports · 2019Trial
- Safety and Metabolism of Long-term Administration of NIAGEN (Nicotinamide Riboside Chloride) in a Randomized, Double-Blind, Placebo-controlled Clinical Trial of Healthy Overweight Adults.Scientific reports · 2019Trial
- Chronic nicotinamide riboside supplementation is well-tolerated and elevates NADNature communications · 2018Trial
- Based on metabolome and transcriptome analysis, differences in organic acids of 'Korla Xiangli' fruit from different male parent.Food chemistry. Molecular sciences · 2026Article
- Beyond deacetylation: crosstalk mechanisms and context-dependent regulation of sirtuin non-classical enzymatic functions in disease.Molecular medicine (Cambridge, Mass.) · 2026Review
- Review
- Population-scale characterization of the oral microbiome and associations with metabolic health.Nature communications · 2026Article
- The effect of obesity and aging on NADInternational journal of obesity (2005) · 2026Article
- Simultaneous Detection and Quantification of Adenine Nucleotides in Mammalian Cultured Cells by HPLC.ACS omega · 2026Article
- Nicotinamide salvage is required for proliferation and sustaining self-renewal in undifferentiated embryonic stem cells.Stem cells (Dayton, Ohio) · 2026Article
- Glycerol-3-phosphate activates ChREBP, FGF21 transcription and lipogenesis in citrin deficiency.Nature metabolism · 2025Article
- Modulating inter-mitochondrial contacts to increase membrane potential for mitigating blue light damage.bioRxiv : the preprint server for biology · 2025Article
- Multiplexed Targeted Spatial Mass Spectrometry Imaging Assays to monitor lipids and NADResearch square · 2025Article
- A mixed-mode LC-MS-based method for comprehensive analysis of NAD and related metabolites from biological sample matrices.Scientific reports · 2025Article
- NADK as a molecular marker to distinguish between alcohol- and non-alcohol-associated liver cirrhosis: A pilot study.Clinical and experimental hepatology · 2025Article
- NAD World 3.0: the importance of the NMN transporter and eNAMPT in mammalian aging and longevity control.npj aging · 2025Review
- Whole Blood Metabolomic Profiling of Mice with Tacrolimus-Induced Chronic Nephrotoxicity: NADAntioxidants (Basel, Switzerland) · 2025Article
- Metabolic Pathway Tracing for NADMethods in molecular biology (Clifton, N.J.) · 2025Article
- Zic-HILIC MS/MS Method for NADomics Provides Novel Insights into Redox Homeostasis inMetabolites · 2024Article
57 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Nicotinamide adenine dinucleotide (NAD(+)) is a coenzyme for hydride transfer reactions and a substrate for sirtuins and other NAD(+)-consuming enzymes. The abundance of NAD (+), NAD(+) biosynthetic intermediates, and related nucleotides reflects the metabolic state of cells and tissues. High performance liquid chromatography (HPLC) followed by ultraviolet-visible (UV-Vis) spectroscopic analysis of NAD(+) metabolites does not offer the specificity and sensitivity necessary for robust quantification of complex samples. Thus, we developed a targeted, quantitative assay of the NAD(+) metabolome with the use of HPLC coupled to mass spectrometry. Here we discuss NAD(+) metabolism as well as the technical challenges required for reliable quantification of the NAD(+) metabolites. The new method incorporates new separations and improves upon a previously published method that suffered from the problem of ionization suppression for particular compounds.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.