ReviewComputational and structural biotechnology journal2012
Directed evolution: selection of the host organism.
Review in Computational and structural biotechnology journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed.
- Harnessing the central dogma: Enzyme-driven gene diversification strategies for in cellulo continuous directed evolution.Fundamental research · 2026Review
- S-SELeCT: a human-evolved serine integrase system for efficient large-cargo genome integration.Nucleic acids research · 2026Article
- Precise, minimally evolved adenine base editors generated through mutation reversion analysis.Nature biotechnology · 2026Article
- Generating Site Saturation Mutagenesis Libraries and Transferring Them to Broad Host-Range Plasmids Using Golden Gate Cloning.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Fungal Laccases: Fundamentals, Engineering and Classification Update.Biomolecules · 2023Review
- Clonal Amplification-Enhanced Gene Expression in Synthetic Vesicles.ACS synthetic biology · 2023Article
- Rapid directed molecular evolution of fluorescent proteins in mammalian cells.Protein science : a publication of the Protein Society · 2022Article
- Insufficiency in functional genomics studies, data, and applications: A case study of bio-prospecting research in ruminant microbiome.Frontiers in genetics · 2022Review
- Learning Strategies in Protein Directed Evolution.Methods in molecular biology (Clifton, N.J.) · 2022Review
- Novel Cold-Adapted Recombinant Laccase KbLcc1 fromInternational journal of molecular sciences · 2021Article
- Screening Collagenase Activity in Bacterial Lysate for Directed Enzyme Applications.International journal of molecular sciences · 2021Article
- Directed evolution in mammalian cells.Nature methods · 2021Review
- Grand Challenges in Microalgae Domestication.Frontiers in plant science · 2021Article
- Identification and Expression of New Unspecific Peroxygenases - Recent Advances, Challenges and Opportunities.Frontiers in bioengineering and biotechnology · 2021Review
- Directed evolution of the aryl-alcohol oxidase: Beyond the lab bench.Computational and structural biotechnology journal · 2020Review
- High-throughput droplet-based microfluidics for directed evolution of enzymes.Electrophoresis · 2019Review
- Engineering a microbial platform for de novo biosynthesis of diverse methylxanthines.Metabolic engineering · 2016Article
- Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening.Journal of visualized experiments : JoVE · 2016Article
- Development of simple random mutagenesis protocol for the protein expression system in Pichia pastoris.Biotechnology for biofuels · 2016Article
- Focused Directed Evolution of Aryl-Alcohol Oxidase in Saccharomyces cerevisiae by Using Chimeric Signal Peptides.Applied and environmental microbiology · 2015Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Directed evolution has become a well-established tool for improving proteins and biological systems. A critical aspect of directed evolution is the selection of a suitable host organism for achieving functional expression of the target gene. To date, most directed evolution studies have used either Escherichia coli or Saccharomyces cerevisiae as a host; however, other bacterial and yeast species, as well as mammalian and insect cell lines, have also been successfully used. Recent advances in synthetic biology and genomics have opened the possibility of expanding the use of directed evolution to new host organisms such as microalgae. This review focuses on the different host organisms used in directed evolution and highlights some of the recent directed evolution strategies used in these organisms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.