Evidence map›Paper›PMID 24688520›Full record

ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2013

Use of the VITEK 2 system to identify and test the antifungal susceptibility of clinically relevant yeast species.

M S C Melhem, A Bertoletti, H R L Lucca, R B O Silva, F A Meneghin, M W Szeszs

Abstract readComparative StudyEvaluation Study
In one paragraph

Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. The Antimicrobial Resistance ofJournal of fungi (Basel, Switzerland) · 2025
    Article
  3. Article
  4. Fungal coinfection/superinfection in COVID-19 patients in a tertiary hospital in MexicoBiomedica : revista del Instituto Nacional de Salud · 2024
    Article
  5. First report of amphotericin B resistantIranian journal of microbiology · 2024
    Article
  6. EnhancedCurrent medical mycology · 2023
    Article
  7. Article
  8. High Prevalence ofVeterinary sciences · 2022
    Article
  9. Article
  10. Article
  11. Virulence Factors and Antifungal Susceptibility Profile ofJournal of fungi (Basel, Switzerland) · 2021
    Article
  12. Article
  13. [Identification and susceptibility profile ofRevista panamericana de salud publica = Pan American journal of public health · 2020
    Article
  14. The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2019
    Article
  15. Article
  16. SUSCEPTIBILITY TEST FOR FUNGI: CLINICAL AND LABORATORIAL CORRELATIONS IN MEDICAL MYCOLOGY.Revista do Instituto de Medicina Tropical de Sao Paulo · 2015
    Review
  17. Identification and Antifungal Susceptibility Testing ofJournal of global infectious diseases
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

M S C MelhemInstituto Adolfo Lutz, Secretaria da Saúde, Governo do Estado de São Paulo, São Paulo, SP, Brazil.
A BertolettiInstituto Adolfo Lutz, Secretaria da Saúde, Governo do Estado de São Paulo, São Paulo, SP, Brazil.
H R L LuccaInstituto Adolfo Lutz, Secretaria da Saúde, Governo do Estado de São Paulo, São Paulo, SP, Brazil.
R B O SilvaInstituto Adolfo Lutz, Secretaria da Saúde, Governo do Estado de São Paulo, São Paulo, SP, Brazil.
F A MeneghinInstituto Adolfo Lutz, Secretaria da Saúde, Governo do Estado de São Paulo, São Paulo, SP, Brazil.
M W SzeszsInstituto Adolfo Lutz, Secretaria da Saúde, Governo do Estado de São Paulo, São Paulo, SP, Brazil.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Eleven quality control isolates (Candida albicans ATCC 64548, C. tropicalis ATCC 200956, C. glabrata ATCC 90030, C. lusitaniae ATCC 200951, C. parapsilosis ATCC 22019, C. krusei ATCC 6258, C. dubliniensis ATCC 6330, Saccharomyces cerevisiae ATCC 9763, Cryptococcus neoformans ATCC 90012, C. gattii FIOCRUZ-CPF 60, and Trichosporon mucoides ATCC 204094) and 32 bloodstream isolates, including C. albicans, C. tropicalis, C. parapsilosis, C. glabrata, C. krusei, C. guilliermondii, C. pelliculosa (Pichia anomala), C. haemulonii, C. lusitaniae, and C. kefyr were identified at the species level by the VITEK 2 system. A set of clinical isolates (32 total) were used as challenge strains to evaluate the ability of the VITEK 2 system to determine the antifungal susceptibility of yeasts compared with the CLSI and EUCAST BMD reference standards. The VITEK 2 system correctly identified 100% of the challenge strains. The identification of yeast species and the evaluation of their susceptibility profiles were performed in an automated manner by the VITEK 2 system after approximately 15 h of growth for most species of Candida. The VITEK 2 system ensures that each test is performed in a standardized manner and provides quantitative MIC results that are reproducible and accurate when compared with the BMD reference methods. This system was able to determine the MICs of amphotericin B, flucytosine, voriconazole, and fluconazole in 15 h or less for the most common clinically relevant Candida species. In addition, the VITEK 2 system could reliably identify resistance to flucytosine, voriconazole, and fluconazole and exhibits excellent quantitative and qualitative agreement with the CLSI or EUCAST broth microdilution reference methods.

Indexed as

Antifungal AgentsHumansMicrobial Sensitivity TestsMycosesReproducibility of ResultsTime FactorsYeastsAntifungal Agentsantifungal agentsCandidaCLSIEUCASTMICsusceptibility testing methods

Identifiers

PMID24688520
PMCPMC3958196

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.