Evidence map›Paper›PMID 24688514›Full record

ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2013

Expression of enterovirus 71 capsid protein VP1 in Escherichia coli and its clinical application.

Mei Shi, Yaping Zhou, Limin Cao, Cuijun Ding, Yun Ji, Qinbo Jiang, Xiping Liu, Xiang Li, Xueling Hou, Hongjun Peng and 1 more

Open access · hybridAbstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 9 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 1 institution in 1 country.

Mei ShiDepartment of Clinical Laboratory, The Third Affiliated Hospital of Suzhou University, Changzhou, Jiangsu, P.R. China.
Yaping ZhouChangzhou 21st Century Biotech Research Institute, Changzhou, Jiangsu, P.R. China.
Limin CaoChangzhou 21st Century Biotech Research Institute, Changzhou, Jiangsu, P.R. China.
Cuijun DingDepartment of Clinical Laboratory, Changzhou Children's Hospital, Changzhou, Jiangsu, P.R. China.
Yun JiDepartment of Clinical Laboratory, The Third Affiliated Hospital of Suzhou University, Changzhou, Jiangsu, P.R. China.
Qinbo JiangDepartment of Clinical Laboratory, The Third Affiliated Hospital of Suzhou University, Changzhou, Jiangsu, P.R. China.
Xiping LiuDepartment of Clinical Laboratory, The Third Affiliated Hospital of Suzhou University, Changzhou, Jiangsu, P.R. China.
Xiang LiDepartment of Clinical Laboratory, The Third Affiliated Hospital of Suzhou University, Changzhou, Jiangsu, P.R. China.
Xueling HouDepartment of Clinical Laboratory, The Third Affiliated Hospital of Suzhou University, Changzhou, Jiangsu, P.R. China.
Hongjun PengDepartment of Clinical Laboratory, The Third Affiliated Hospital of Suzhou University, Changzhou, Jiangsu, P.R. China.
Weifeng ShiDepartment of Clinical Laboratory, The Third Affiliated Hospital of Suzhou University, Changzhou, Jiangsu, P.R. China.
Soochow University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The VPl gene of enterovirus 71 (EV71) was synthesized, construct a recombinant plasmid pET15b/VP1 and expressed in E. coli BL21. The recombinant VP1 protein could specifically react with EV71-infected patient sera without the cross-reaction with serum antibodies of coxsackievirus A16 (CA16), A4, A5, B3 and B5 as well as echovirus 6. In acute and convalescent phases, IgM and IgG antibodies of 182 serum samples were detected by ELISA with recombinant VP1 protein as a coated antigen. The results showed that the sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of IgM antibodies in serum samples for the diagnosis of EV71 infection were 90.1, 98.4, 98.8 and 88.7%, respectively; similarly, those of IgG antibodies in serum samples were 82.4, 89.1, 91.5 and 78.1%, respectively. Five of 80 samples (6.25%) from CA16-infected patients were detected positive by ELISA with recombinant VP1 protein in which indicated the cross reactions and 0 of 5 samples from patients infected with other enteroviruses including CA4, CA5, CB3, CB5 and echovirus 6. Therefore, the recombinant VP1 protein of EV7l may provide a theoretical reference for establishing an effective antibody screening of IgM for EV71-infected patients with clinically suspected hand, foot, and mouth disease (HFMD).

Indexed as

Capsid ProteinsAntibodies, ViralChild, PreschoolCloning, MolecularEnterovirus A, HumanEnzyme-Linked Immunosorbent AssayEscherichia coliFemaleGene ExpressionHand, Foot and Mouth DiseaseHumansImmunoglobulin GImmunoglobulin MInfantMalePredictive Value of TestsAntibodies, ViralCapsid ProteinsImmunoglobulin GImmunoglobulin MRecombinant ProteinsELISAEnterovirus 71Gene cloningRecombinant VPl protein

Identifiers

PMID24688514
PMCPMC3958190
OpenAlexW2020993953

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.