ArticleInternational journal of medical sciences2014
Comparison of methods for the extraction of DNA from formalin-fixed, paraffin-embedded archival tissues.
Article in International journal of medical sciences, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed.
- Archived medical specimens as antemortem DNA references for disaster victim identification, optimization study following the October 7 massacre.International journal of legal medicine · 2026Article
- Correlation betweenJournal of dentistry (Shiraz, Iran) · 2025Article
- DNA extraction from long-term preserved formalin-fixed paraffin-embedded samples of thymic epithelial tumours - a performance comparison of three commercial kits.Contemporary oncology (Poznan, Poland) · 2025Article
- The quality of DNA isolated from autopsy formalin-fixed and formalin-fixed paraffin-embedded tissues: study of 1662 samples.Molecular biology reports · 2023Article
- The Role of theBiomedicines · 2023Article
- The ideal reporting ofPathologica · 2023Review
- Molecular Phylogenetic Analysis ofMicroorganisms · 2023Article
- Modelling clinical DNA fragmentation in the development of universal PCR-based assays for bisulfite-converted, formalin-fixed and cell-free DNA sample analysis.Scientific reports · 2022Article
- Comparison Approach for Identifying Missed Invasive Fungal Infections in Formalin-Fixed, Paraffin-Embedded Autopsy Specimens.Journal of fungi (Basel, Switzerland) · 2022Article
- Clinicopathologic Analysis of Sinonasal Inverted Papilloma, with Focus on Human Papillomavirus Infection Status.Diagnostics (Basel, Switzerland) · 2022Article
- Practical tips to using formalin-fixed paraffin-embedded tissue archives for molecular diagnostics in a South African setting.African journal of laboratory medicine · 2022Article
- Article
- Comparison of diagnostic methods for Tetracapsuloides bryosalmonae detection in salmonid fish.Journal of fish diseases · 2021Article
- DNA isolated from formalin-fixed paraffin-embedded healthy tissue after 30 years of storage can be used for forensic studies.Forensic science, medicine, and pathology · 2021Article
- Regulation of dimethylarginine dimethylaminohydrolase 2 expression by NF-κB acetylation.Experimental and therapeutic medicine · 2021Article
- A novel approach for extracting DNA from formalin-fixed paraffin-embedded tissue using microwave.Medical journal, Armed Forces India · 2020Article
- NematodeInternational journal for parasitology. Parasites and wildlife · 2020Article
- Effect of Different Proteinase K Digest Protocols and Deparaffinization Methods on Yield and Integrity of DNA Extracted From Formalin-fixed, Paraffin-embedded Tissue.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2020Article
- Article
- DNA extraction from archived hematoxylin and eosin-stained tissue slides for downstream molecular analysis.World journal of methodology · 2019Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimDiscussing a protocol involving xylene-ethanol deparaffinization on slides followed by a kit-based extraction that allows for the extraction of high quality DNA from FFPE tissues.
methodsDNA was extracted from the FFPE tissues of 16 randomly selected blocks. Methods involving deparaffinization on slides or tubes, enzyme digestion overnight or for 72 hours and isolation using phenol chloroform method or a silica-based commercial kit were compared in terms of yields, concentrations and the amplifiability.
resultsThe highest yield of DNA was produced from the samples that were deparaffinized on slides, digested for 72 hours and isolated with a commercial kit. Samples isolated with the phenol-chloroform method produced DNA of lower purity than the samples that were purified with kit. The samples isolated with the commercial kit resulted in better PCR amplification.
conclusionSilica-based commercial kits and deparaffinized on slides should be considered for DNA extraction from FFPE.
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