Evidence map›Paper›PMID 24688174›Full record

ArticleCanadian journal of veterinary research = Revue canadienne de recherche veterinaire2014

Porcine salivary analysis by 2-dimensional gel electrophoresis in 3 models of acute stress: a pilot study.

María Fuentes-Rubio, José J Cerón, Carlos de Torre, Damián Escribano, Ana M Gutiérrez, Fernando Tecles

Open access · greenAbstract read
In one paragraph

Article in Canadian journal of veterinary research = Revue canadienne de recherche veterinaire, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.0field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 11 citations in OpenAlex.

  1. Article
  2. Welfare Assessment in Pigs Using the Salivary Proteome.Animals : an open access journal from MDPI · 2024
    Review
  3. Article
  4. Article
  5. Article
  6. Validation of an assay for quantification of alpha-amylase in saliva of sheep.Canadian journal of veterinary research = Revue canadienne de recherche veterinaire · 2016
    Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

María Fuentes-RubioDepartment of Animal Medicine and Surgery, Regional Campus of International Excellence Campus Mare Nostrum, University of Murcia, 30100, Espinardo, Murcia, Spain (Fuentes-Rubio, Cerón, Escribano, Gutiérrez, Tecles); Proteomics Unit, University Hospital Virgen de la Arrixaca, Ctra Madrid-Cartagena s/n 30120, El Palmar-Murcia, Spain (de Torre).
José J CerónDepartment of Animal Medicine and Surgery, Regional Campus of International Excellence Campus Mare Nostrum, University of Murcia, 30100, Espinardo, Murcia, Spain (Fuentes-Rubio, Cerón, Escribano, Gutiérrez, Tecles); Proteomics Unit, University Hospital Virgen de la Arrixaca, Ctra Madrid-Cartagena s/n 30120, El Palmar-Murcia, Spain (de Torre).
Carlos de TorreDepartment of Animal Medicine and Surgery, Regional Campus of International Excellence Campus Mare Nostrum, University of Murcia, 30100, Espinardo, Murcia, Spain (Fuentes-Rubio, Cerón, Escribano, Gutiérrez, Tecles); Proteomics Unit, University Hospital Virgen de la Arrixaca, Ctra Madrid-Cartagena s/n 30120, El Palmar-Murcia, Spain (de Torre).
Damián EscribanoDepartment of Animal Medicine and Surgery, Regional Campus of International Excellence Campus Mare Nostrum, University of Murcia, 30100, Espinardo, Murcia, Spain (Fuentes-Rubio, Cerón, Escribano, Gutiérrez, Tecles); Proteomics Unit, University Hospital Virgen de la Arrixaca, Ctra Madrid-Cartagena s/n 30120, El Palmar-Murcia, Spain (de Torre).
Ana M GutiérrezDepartment of Animal Medicine and Surgery, Regional Campus of International Excellence Campus Mare Nostrum, University of Murcia, 30100, Espinardo, Murcia, Spain (Fuentes-Rubio, Cerón, Escribano, Gutiérrez, Tecles); Proteomics Unit, University Hospital Virgen de la Arrixaca, Ctra Madrid-Cartagena s/n 30120, El Palmar-Murcia, Spain (de Torre).
Fernando TeclesDepartment of Animal Medicine and Surgery, Regional Campus of International Excellence Campus Mare Nostrum, University of Murcia, 30100, Espinardo, Murcia, Spain (Fuentes-Rubio, Cerón, Escribano, Gutiérrez, Tecles); Proteomics Unit, University Hospital Virgen de la Arrixaca, Ctra Madrid-Cartagena s/n 30120, El Palmar-Murcia, Spain (de Torre).
Hospital Universitario Virgen de la Arrixaca · ES

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The purpose of this research was to study changes in the salivary proteome of healthy pigs in stressful situations to identify any potential new salivary biomarker of stress. Three groups of animals were subjected to 3 stress models: snaring restraint followed by simulated sampling of vena cava blood; brief transport by road; and restriction of movement in a digestibility cage. Saliva was obtained from each animal before and 15 and 30 min after the induction of stress. The samples from the animals that showed the greatest increase in salivary cortisol concentration were pooled and run on 2-dimensional gels. Coomassie Brilliant Blue R-250 was used for spot detection and mass spectrometry for spot identification. Statistical analyses showed that 2 proteins had significant differences in expression before and after the induction of stress. These proteins were identified as odorant-binding protein and fragments of albumin. Further studies will be necessary to confirm the value of using these proteins as salivary biomarkers of stress in pigs.

Indexed as

AnimalsBiomarkersElectrophoresis, Gel, Two-DimensionalHydrocortisonePilot ProjectsProteomeSalivaStress, PhysiologicalSwineTandem Mass SpectrometryBiomarkersHydrocortisoneProteome

Identifiers

PMID24688174
PMCPMC3962275
OpenAlexW2133863433

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.