ArticleThe Journal of infectious diseases2014
Hemoglobinopathic erythrocytes affect the intraerythrocytic multiplication of Plasmodium falciparum in vitro.
Article in The Journal of infectious diseases, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 28 citations in OpenAlex.
- G6PD variants but not Filipino beta-thalassemia are associated with reduced risk of Plasmodium knowlesi infection in Sabah, Malaysia.Malaria journal · 2026Article
- Microfluidic platform for automatic quantification of malaria parasite invasion under physiological flow conditions.Lab on a chip · 2026Article
- Kinetics of antimalarial antibodies in children with common haemoglobinopathies in a Tanzanian population.Frontiers in immunology · 2026Article
- Under conditions of high wall shear stress, several PfEBA and PfRH ligands are important for malariamBio · 2025Article
- Application of optical tweezer technology reveals that PfEBA and PfRH ligands, not PfMSP1, play a central role in Plasmodium falciparum merozoite-erythrocyte attachment.PLoS pathogens · 2024Article
- [Blood count abnormalities in the association of sickle cell disease and malaria in clinical hematology at the CNHU-HKM in Cotonou (Bénin)].Medecine tropicale et sante internationale · 2024Article
- Cultivation of Asexual Intraerythrocytic Stages ofPathogens (Basel, Switzerland) · 2023Review
- Plasmodium schizogony, a chronology of the parasite's cell cycle in the blood stage.PLoS pathogens · 2023Review
- Elucidating parasite and host-cell factors enabling Babesia infection in sickle red cells under hypoxic/hyperoxic conditions.Blood advances · 2023Article
- Novel stem cell technologies are powerful tools to understand the impact of human factors onFrontiers in cellular and infection microbiology · 2023Article
- Red Blood Cells Oligosaccharides as Targets for Plasmodium Invasion.Biomolecules · 2022Review
- The erythrocyte membrane properties of beta thalassaemia heterozygotes and their consequences for Plasmodium falciparum invasion.Scientific reports · 2022Article
- From marginal to essential: the golden thread between nutrient sensing, medium composition and Plasmodium vivax maturation in in vitro culture.Malaria journal · 2019Review
- EXP2 is a nutrient-permeable channel in the vacuolar membrane of Plasmodium and is essential for protein export via PTEX.Nature microbiology · 2018Article
- Validity of simple clinical and biological parameters as screening tool for sickle cell anemia for referral to tertiary center in highly resource constraints.Journal of clinical laboratory analysis · 2017Article
- Oxidative insult can induce malaria-protective trait of sickle and fetal erythrocytes.Nature communications · 2016Article
- Article
- Hemoglobin S and C affect protein export in Plasmodium falciparum-infected erythrocytes.Biology open · 2015Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
Abstract
backgroundThe mechanisms by which α-thalassemia and sickle cell traits confer protection from severe Plasmodium falciparum malaria are not yet fully elucidated. We hypothesized that hemoglobinopathic erythrocytes reduce the intraerythrocytic multiplication of P. falciparum, potentially delaying the development of life-threatening parasite densities until parasite clearing immunity is achieved.
methodsWe developed a novel in vitro assay to quantify the number of merozoites released from an individual schizont, termed the "intraerythrocytic multiplication factor" (IMF).
resultsP. falciparum (3D7 line) schizonts produce variable numbers of merozoites in all erythrocyte types tested, with median IMFs of 27, 27, 29, 23, and 23 in control, HbAS, HbSS, and α- and β-thalassemia trait erythrocytes, respectively. IMF correlated strongly (r(2) = 0.97; P < .001) with mean corpuscular hemoglobin concentration, and varied significantly with mean corpuscular volume and hemoglobin content. Reduction of IMFs in thalassemia trait erythrocytes was confirmed using clinical parasite isolates with different IMFs. Mathematical modeling of the effect of IMF on malaria progression indicates that the lower IMF in thalassemia trait erythrocytes limits parasite density and anemia severity over the first 2 weeks of parasite replication.
conclusionsP. falciparum IMF, a parasite heritable virulence trait, correlates with erythrocyte indices and is reduced in thalassemia trait erythrocytes. Parasite IMF should be examined in other low-indices erythrocytes.
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