ArticleAntimicrobial agents and chemotherapy2014
Discovery of novel cell wall-active compounds using P ywaC, a sensitive reporter of cell wall stress, in the model gram-positive bacterium Bacillus subtilis.
Article in Antimicrobial agents and chemotherapy, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- A double-layer plate method for rapid screening of cell wall-targeting antibiotic producers using a microbial whole-cell biosensor.Applied and environmental microbiology · 2026Article
- Bacillus subtilis uses the SigM signaling pathway to prioritize the use of its lipid carrier for cell wall synthesis.PLoS biology · 2024Article
- An extensively validated whole-cell biosensor for specific, sensitive and high-throughput detection of antibacterial inhibitors targeting cell-wall biosynthesis.The Journal of antimicrobial chemotherapy · 2023Article
- Development of Whole-Cell Biosensors for Screening of Peptidoglycan-Targeting Antibiotics in a Gram-Negative Bacterium.Applied and environmental microbiology · 2022Article
- Crosstalk between guanosine nucleotides regulates cellular heterogeneity in protein synthesis during nutrient limitation.PLoS genetics · 2022Article
- Inhibition ofMicrobiology (Reading, England) · 2022Article
- Development of a whole-cell biosensor for detection of antibiotics targeting bacterial cell envelope in Bacillus subtilis.Applied microbiology and biotechnology · 2022Article
- A genetic screen to identify factors affected by undecaprenyl phosphate recycling uncovers novel connections to morphogenesis in Escherichia coli.Molecular microbiology · 2021Article
- Constitutive expression of the cryptic vanGCd operon promotes vancomycin resistance in Clostridioides difficile clinical isolates.The Journal of antimicrobial chemotherapy · 2020Article
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- Amphotericin B Specifically Induces the Two-Component System LnrJK: Development of a Novel Whole-Cell Biosensor for the Detection of Amphotericin-Like Polyenes.Frontiers in microbiology · 2020Article
- A Chemical Inhibitor of Cell Growth Reduces Cell Size in Bacillus subtilis.ACS chemical biology · 2019Article
- Induction of the Spx regulon by cell wall stress reveals novel regulatory mechanisms in Bacillus subtilis.Molecular microbiology · 2018Article
- Diamide Inhibitors of the Bacillus subtilis N-Acetylglucosaminidase LytG That Exhibit Antibacterial Activity.ACS infectious diseases · 2017Article
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- Article
- Bacillus subtilis extracytoplasmic function (ECF) sigma factors and defense of the cell envelope.Current opinion in microbiology · 2016Review
- The Canadian Institutes of Health Research response to antimicrobial resistance.Canada communicable disease report = Releve des maladies transmissibles au Canada · 2015Article
- RelA inhibits Bacillus subtilis motility and chaining.Journal of bacteriology · 2015Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
The emergence of antibiotic resistance in recent years has radically reduced the clinical efficacy of many antibacterial treatments and now poses a significant threat to public health. One of the earliest studied well-validated targets for antimicrobial discovery is the bacterial cell wall. The essential nature of this pathway, its conservation among bacterial pathogens, and its absence in human biology have made cell wall synthesis an attractive pathway for new antibiotic drug discovery. Herein, we describe a highly sensitive screening methodology for identifying chemical agents that perturb cell wall synthesis, using the model of the Gram-positive bacterium Bacillus subtilis. We report on a cell-based pilot screen of 26,000 small molecules to look for cell wall-active chemicals in real time using an autonomous luminescence gene cluster driven by the promoter of ywaC, which encodes a guanosine tetra(penta)phosphate synthetase that is expressed under cell wall stress. The promoter-reporter system was generally much more sensitive than growth inhibition testing and responded almost exclusively to cell wall-active antibiotics. Follow-up testing of the compounds from the pilot screen with secondary assays to verify the mechanism of action led to the discovery of 9 novel cell wall-active compounds.
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