ArticleThe Journal of cell biology2014
Ensconsin/Map7 promotes microtubule growth and centrosome separation in Drosophila neural stem cells.
Article in The Journal of cell biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
37 citing papers in PubMed, 69 citations in OpenAlex.
- The role of chromosome/kinetochore-driven microtubule generation in Drosophila spindle assembly.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2025Review
- Positioning centrioles and centrosomes.The Journal of cell biology · 2024Review
- A structural and dynamic visualization of the interaction between MAP7 and microtubules.Nature communications · 2024Article
- Imaging and Analysis of Drosophila Neural Stem Cell Asymmetric Division.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Article
- Resonance assignments of the microtubule-binding domain of the microtubule-associated protein 7 (MAP7).Biomolecular NMR assignments · 2023Article
- Microtubule-associated proteins MAP7 and MAP7D1 promote DNA double-strand break repair in the G1 cell cycle phase.iScience · 2023Article
- Pericentrin interacts with Kinesin-1 to drive centriole motility.The Journal of cell biology · 2022Article
- Article
- The spindle assembly checkpoint and the spatial activation of Polo kinase determine the duration of cell division and prevent tumor formation.PLoS genetics · 2022Article
- Structural and functional insight into regulation of kinesin-1 by microtubule-associated protein MAP7.Science (New York, N.Y.) · 2022Article
- Review
- Ensconsin-dependent changes in microtubule organization and LINC complex-dependent changes in nucleus-nucleus interactions result in quantitatively distinct myonuclear positioning defects.Molecular biology of the cell · 2021Article
- A release-and-capture mechanism generates an essential non-centrosomal microtubule array during tube budding.Nature communications · 2021Article
- Mms19 promotes spindle microtubule assembly in Drosophila neural stem cells.PLoS genetics · 2020Article
- Neural innervation as a potential trigger of morphological color change and sexual dimorphism in cichlid fish.Scientific reports · 2020Article
- A Combinatorial MAP Code Dictates Polarized Microtubule Transport.Developmental cell · 2020Article
- Chromosomes function as a barrier to mitotic spindle bipolarity in polyploid cells.The Journal of cell biology · 2020Article
- MYC Dysregulates Mitosis, Revealing Cancer Vulnerabilities.Cell reports · 2020Article
- MAP7 promotes migration and invasion and progression of human cervical cancer through modulating the autophagy.Cancer cell international · 2020Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 3 countries.
Funding
Abstract
The mitotic spindle is crucial to achieve segregation of sister chromatids. To identify new mitotic spindle assembly regulators, we isolated 855 microtubule-associated proteins (MAPs) from Drosophila melanogaster mitotic or interphasic embryos. Using RNAi, we screened 96 poorly characterized genes in the Drosophila central nervous system to establish their possible role during spindle assembly. We found that Ensconsin/MAP7 mutant neuroblasts display shorter metaphase spindles, a defect caused by a reduced microtubule polymerization rate and enhanced by centrosome ablation. In agreement with a direct effect in regulating spindle length, Ensconsin overexpression triggered an increase in spindle length in S2 cells, whereas purified Ensconsin stimulated microtubule polymerization in vitro. Interestingly, ensc-null mutant flies also display defective centrosome separation and positioning during interphase, a phenotype also detected in kinesin-1 mutants. Collectively, our results suggest that Ensconsin cooperates with its binding partner Kinesin-1 during interphase to trigger centrosome separation. In addition, Ensconsin promotes microtubule polymerization during mitosis to control spindle length independent of Kinesin-1.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.