ArticleJournal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry2014
Cognate and noncognate metal ion coordination in metal-specific metallothioneins: the Helix pomatia system as a model.
Article in Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 30 citations in OpenAlex.
- Identification of two metallothioneins in Agaricus crocodilinus reveals gene duplication and domain expansion, a pattern conserved across fungal species.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2025Article
- A multi-domain snail metallothionein increases cadmium resistance and fitness in Caenorhabditis elegans.Scientific reports · 2024Article
- Metals and metallothionein evolution in snails: a contribution to the concept of metal-specific functionality from an animal model group.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2024Review
- Evolution of Cd2+ and Cu+ binding in Helix pomatia metallothioneins.Metallomics : integrated biometal science · 2023Article
- A de novo evolved domain improves the cadmium detoxification capacity of limpet metallothioneins.Scientific reports · 2023Article
- Study on metal binding capacity of the freshwater crab Sinopotamon henanense's recombinant copper specific binding metallothionein expressed in Escherichia coli.Ecotoxicology (London, England) · 2022Article
- Metal-Specificity Divergence between Metallothioneins ofInternational journal of molecular sciences · 2021Article
- Tunicates Illuminate the Enigmatic Evolution of Chordate Metallothioneins by Gene Gains and Losses, Independent Modular Expansions, and Functional Convergences.Molecular biology and evolution · 2021Article
- Modularity in Protein Evolution: Modular Organization and De Novo Domain Evolution in Mollusk Metallothioneins.Molecular biology and evolution · 2021Article
- Two Unconventional Metallothioneins in the Apple SnailInternational journal of molecular sciences · 2020Article
- Cadmium Uptake,International journal of molecular sciences · 2020Article
- Isolation and Characterization of Copper- and Zinc- Binding Metallothioneins from the Marine AlgaInternational journal of molecular sciences · 2019Article
- Differential reactivity of closely related zinc(II)-binding metallothioneins from the plant Arabidopsis thaliana.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2018Article
- Unravelling the Role of Metallothionein on Development, Reproduction and Detoxification in the Wall Lizard Podarcis sicula.International journal of molecular sciences · 2017Review
- Biomphalaria glabrata Metallothionein: Lacking Metal Specificity of the Protein and Missing Gene Upregulation Suggest Metal Sequestration by Exchange Instead of through Selective Binding.International journal of molecular sciences · 2017Article
- Analysis of Metal-Binding Features of the Wild Type and Two Domain-Truncated Mutant Variants of Littorina littorea Metallothionein Reveals Its Cd-Specific Character.International journal of molecular sciences · 2017Article
- Cadmium Protection Strategies--A Hidden Trade-Off?International journal of molecular sciences · 2016Review
- Earthworm Lumbricus rubellus MT-2: Metal Binding and Protein Folding of a True Cadmium-MT.International journal of molecular sciences · 2016Article
- Does Variation of the Inter-Domain Linker Sequence Modulate the Metal Binding Behaviour of Helix pomatia Cd-Metallothionein?International journal of molecular sciences · 2015Article
- Hints for metal-preference protein sequence determinants: different metal binding features of the five tetrahymena thermophila metallothioneins.International journal of biological sciences · 2015Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
The Helix pomatia metallothionein (MT) system, namely, its two highly specific forms, HpCdMT and HpCuMT, has offered once again an optimum model to study metal-protein specificity. The present work investigates the most unexplored aspect of the coordination behavior of MT polypeptides with respect to either cognate or noncognate metal ions, as opposed to the standard studies of cognate metal ion coordination. To this end, we analyzed the in vivo synthesis of the corresponding complexes with their noncognate metals, and we performed a detailed spectroscopic and spectrometric study of the Zn(2+)/Cd(2+) and Zn(2+)/Cu(+) in vitro replacement reactions on the initial Zn-HpMT species. An HpCuMTAla site-directed mutant, exhibiting differential Cu(+)-binding abilities in vivo, was also included in this study. We demonstrate that when an MT binds its cognate metal, it yields well-folded complexes of limited stoichiometry, representative of minimal-energy conformations. In contrast, the incorporation of noncognate metal ions is better attributed to an unspecific reaction of cysteinic thiolate groups with metal ions, which is dependent on their concentration in the surrounding milieu, where no minimal-energy structure is reached, and otherwise, the MT peptide acts as a multidentate ligand that will bind metal ions until its capacity has been saturated. Additionally, we suggest that previous binding of an MT polypeptide with its noncognate metal ion (e.g., binding of Zn(2+) to the HpCuMT isoform) may preclude the correct folding of the complex with its cognate metal ion.
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