ArticleJournal of visualized experiments : JoVE2014
Imaging cell membrane injury and subcellular processes involved in repair.
Article in Journal of visualized experiments : JoVE, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
30 citing papers in PubMed, 47 citations in OpenAlex.
- Article
- Glycative stress inhibits hypertrophy and impairs cell membrane integrity in overloaded mouse skeletal muscle.Journal of cachexia, sarcopenia and muscle · 2024Article
- Early Endosomes Undergo Calcium-Triggered Exocytosis and Enable Repair of Diffuse and Focal Plasma Membrane Injury.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Early Endosomes Act as Local Exocytosis Hubs to Repair Endothelial Membrane Damage.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Monitoring Plasma Membrane Injury-Triggered Endocytosis at Single-Cell and Single-Vesicle Resolution.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Secreted acid sphingomyelinase as a potential gene therapy for limb girdle muscular dystrophy 2B.The Journal of clinical investigation · 2022Article
- The resealing factor S100A11 interacts with annexins and extended synaptotagmin-1 in the course of plasma membrane wound repair.Frontiers in cell and developmental biology · 2022Article
- Endoplasmic reticulum maintains ion homeostasis required for plasma membrane repair.The Journal of cell biology · 2021Article
- Mitochondrial dysfunction and consequences in calpain-3-deficient muscle.Skeletal muscle · 2020Article
- Article
- Mitochondrial fragmentation enables localized signaling required for cell repair.The Journal of cell biology · 2020Article
- Zinc Oxide Nanocrystals and High-Energy Shock Waves: A New Synergy for the Treatment of Cancer Cells.Frontiers in bioengineering and biotechnology · 2020Article
- Dysregulation of Mitochondrial CaCell reports · 2019Article
- Mitochondrial dysfunction and role of harakiri in the pathogenesis of myositis.The Journal of pathology · 2019Article
- Transmission of Mechanical Information by Purinergic Signaling.Biophysical journal · 2019Article
- High-throughput Measurement of Plasma Membrane Resealing Efficiency in Mammalian Cells.Journal of visualized experiments : JoVE · 2019Article
- Dysregulated calcium homeostasis prevents plasma membrane repair in Anoctamin 5/TMEM16E-deficient patient muscle cells.Cell death discovery · 2019Article
- Membrane Stabilization by Modified Steroid Offers a Potential Therapy for Muscular Dystrophy Due to Dysferlin Deficit.Molecular therapy : the journal of the American Society of Gene Therapy · 2018Article
- Multiple Mechanisms Drive Calcium Signal Dynamics around Laser-Induced Epithelial Wounds.Biophysical journal · 2017Article
- Mitochondrial redox signaling enables repair of injured skeletal muscle cells.Science signaling · 2017Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The ability of injured cells to heal is a fundamental cellular process, but cellular and molecular mechanisms involved in healing injured cells are poorly understood. Here assays are described to monitor the ability and kinetics of healing of cultured cells following localized injury. The first protocol describes an end point based approach to simultaneously assess cell membrane repair ability of hundreds of cells. The second protocol describes a real time imaging approach to monitor the kinetics of cell membrane repair in individual cells following localized injury with a pulsed laser. As healing injured cells involves trafficking of specific proteins and subcellular compartments to the site of injury, the third protocol describes the use of above end point based approach to assess one such trafficking event (lysosomal exocytosis) in hundreds of cells injured simultaneously and the last protocol describes the use of pulsed laser injury together with TIRF microscopy to monitor the dynamics of individual subcellular compartments in injured cells at high spatial and temporal resolution. While the protocols here describe the use of these approaches to study the link between cell membrane repair and lysosomal exocytosis in cultured muscle cells, they can be applied as such for any other adherent cultured cell and subcellular compartment of choice.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.