ArticleJournal of visualized experiments : JoVE2014
Visualization of the immunological synapse by dual color time-gated stimulated emission depletion (STED) nanoscopy.
Article in Journal of visualized experiments : JoVE, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed, 11 citations in OpenAlex.
- Exploring the Role of Astroglial Glutamate Release and Association With Synapses in Neuronal Function and Behavior.Biological psychiatry · 2018Review
- Visualizing the Actin and Microtubule Cytoskeletons at the B-cell Immune Synapse Using Stimulated Emission Depletion (STED) Microscopy.Journal of visualized experiments : JoVE · 2018Article
- Quantitative Imaging Approaches to Study the CAR Immunological Synapse.Molecular therapy : the journal of the American Society of Gene Therapy · 2017Review
- Analyzing Actin Dynamics at the Immunological Synapse.Methods in molecular biology (Clifton, N.J.) · 2017Article
- High- and Super-Resolution Microscopy Imaging of the NK Cell Immunological Synapse.Methods in molecular biology (Clifton, N.J.) · 2016Article
- Imaging of Cell-Cell Communication in a Vertical Orientation Reveals High-Resolution Structure of Immunological Synapse and Novel PD-1 Dynamics.Journal of immunology (Baltimore, Md. : 1950) · 2015Article
- Super-resolution imaging of the natural killer cell immunological synapse on a glass-supported planar lipid bilayer.Journal of visualized experiments : JoVE · 2015Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Natural killer cells form tightly regulated, finely tuned immunological synapses (IS) in order to lyse virally infected or tumorigenic cells. Dynamic actin reorganization is critical to the function of NK cells and the formation of the IS. Imaging of F-actin at the synapse has traditionally utilized confocal microscopy, however the diffraction limit of light restricts resolution of fluorescence microscopy, including confocal, to approximately 200 nm. Recent advances in imaging technology have enabled the development of subdiffraction limited super-resolution imaging. In order to visualize F-actin architecture at the IS we recapitulate the NK cell cytotoxic synapse by adhering NK cells to activating receptor on glass. We then image proteins of interest using two-color stimulated emission depletion microscopy (STED). This results in <80 nm resolution at the synapse. Herein we describe the steps of sample preparation and the acquisition of images using dual color STED nanoscopy to visualize F-actin at the NK IS. We also illustrate optimization of sample acquisition using Leica SP8 software and time-gated STED. Finally, we utilize Huygens software for post-processing deconvolution of images.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.