ArticleJournal of visualized experiments : JoVE2014
A flow adhesion assay to study leucocyte recruitment to human hepatic sinusoidal endothelium under conditions of shear stress.
Article in Journal of visualized experiments : JoVE, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 31 citations in OpenAlex.
- An In Vitro Model to Study Drugs That Affect Macrophage Adhesion to Murine Brain Endothelial Cells After Proinflammatory Insults of LPS and Pilocarpine.Bio-protocol · 2026Article
- CLEC14A correlates with neutrophil infiltration in hepatocellular carcinoma and mediates neutrophil recruitment across liver endothelial cells.The Journal of pathology · 2026Article
- A deep learning approach to assess transendothelial cell trafficking performance.Scientific reports · 2026Article
- The senescent secretome drives PLVAP expression in cultured human hepatic endothelial cells to promote monocyte transmigration.iScience · 2023Article
- Mechanobiology of the endothelium in vascular health and disease: in vitro shear stress models.Cardiovascular drugs and therapy · 2023Review
- Adhesion to the Brain Endothelium Selects Breast Cancer Cells with Brain Metastasis Potential.International journal of molecular sciences · 2023Article
- Scavenger Receptors: Novel Roles in the Pathogenesis of Liver Inflammation and Cancer.Seminars in liver disease · 2022Review
- Influence of microflow on hepatic sinusoid blood flow and red blood cell deformation.Biophysical journal · 2021Article
- Functional binding of E-selectin to its ligands is enhanced by structural features beyond its lectin domain.The Journal of biological chemistry · 2020Article
- Prognostic Value and Potential Immunoregulatory Role of SCARF1 in Hepatocellular Carcinoma.Frontiers in oncology · 2020Article
- A C-terminal CXCL8 peptide based on chemokine-glycosaminoglycan interactions reduces neutrophil adhesion and migration during inflammation.Immunology · 2019Article
- Article
- More Than Just a Removal Service: Scavenger Receptors in Leukocyte Trafficking.Frontiers in immunology · 2018Review
- SCARF-1 promotes adhesion of CD4Scientific reports · 2017Article
- CD151 supports VCAM-1-mediated lymphocyte adhesion to liver endothelium and is upregulated in chronic liver disease and hepatocellular carcinoma.American journal of physiology. Gastrointestinal and liver physiology · 2017Article
- Human liver sinusoidal endothelial cells promote intracellular crawling of lymphocytes during recruitment: A new step in migration.Hepatology (Baltimore, Md.) · 2017Article
- NOTCH1 mediates a switch between two distinct secretomes during senescence.Nature cell biology · 2016Article
- Intestinal CCL25 expression is increased in colitis and correlates with inflammatory activity.Journal of autoimmunity · 2016Article
- Targeting Tumor Necrosis Factor-α with Adalimumab: Effects on Endothelial Activation and Monocyte Adhesion.PloS one · 2016Article
- Human Cytomegalovirus Up-Regulates Endothelin Receptor Type B: Implication for Vasculopathies?Open forum infectious diseases · 2015Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Leucocyte infiltration into human liver tissue is a common process in all adult inflammatory liver diseases. Chronic infiltration can drive the development of fibrosis and progression to cirrhosis. Understanding the molecular mechanisms that mediate leucocyte recruitment to the liver could identify important therapeutic targets for liver disease. The key interaction during leucocyte recruitment is that of inflammatory cells with endothelium under conditions of shear stress. Recruitment to the liver occurs within the low shear channels of the hepatic sinusoids which are lined by hepatic sinusoidal endothelial cells (HSEC). The conditions within the hepatic sinusoids can be recapitulated by perfusing leucocytes through channels lined by human HSEC monolayers at specific flow rates. In these conditions leucocytes undergo a brief tethering step followed by activation and firm adhesion, followed by a crawling step and subsequent transmigration across the endothelial layer. Using phase contrast microscopy, each step of this 'adhesion cascade' can be visualized and recorded followed by offline analysis. Endothelial cells or leucocytes can be pretreated with inhibitors to determine the role of specific molecules during this process.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.