ReviewCurrent opinion in biotechnology2014
Metabolic flux analysis of Escherichia coli knockouts: lessons from the Keio collection and future outlook.
Review in Current opinion in biotechnology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed.
- Metabolic engineering ofFood chemistry: X · 2026Article
- Dissecting Metabolic Rewiring and Gene-Metabolite Interactions by Utilizing Untargeted Metabolomics and Single-Gene Knockouts in the Model MicroorganismJournal of the American Society for Mass Spectrometry · 2026Article
- An integrated structural and biophysical approach to study carbon metabolism inQRB discovery · 2025Review
- 13 C-MFA helps to identify metabolic bottlenecks for improving malic acid production in Myceliophthora thermophila.Microbial cell factories · 2024Article
- Machine learning identifies key metabolic reactions in bacterial growth on different carbon sources.Molecular systems biology · 2024Article
- Metabolic flux analysis: a comprehensive review on sample preparation, analytical techniques, data analysis, computational modelling, and main application areas.RSC advances · 2022Review
- Exploring the Glucose Fluxotype of theMetabolites · 2021Article
- Article
- From Escherichia coli mutant 13C labeling data to a core kinetic model: A kinetic model parameterization pipeline.PLoS computational biology · 2019Article
- Involvement of multiple influx and efflux transporters in the accumulation of cationic fluorescent dyes by Escherichia coli.BMC microbiology · 2019Article
- Genome-Scale Fluxome ofPlant physiology · 2019Article
- How adaptive evolution reshapes metabolism to improve fitness: recent advances and future outlook.Current opinion in chemical engineering · 2018Article
- Dissecting the genetic and metabolic mechanisms of adaptation to the knockout of a major metabolic enzyme inProceedings of the National Academy of Sciences of the United States of America · 2018Article
- A comprehensive evaluation of constraining amino acid biosynthesis in compartmented models for metabolic flux analysis.Metabolic engineering communications · 2017Article
- Metabolism of the fast-growing bacterium Vibrio natriegens elucidated byMetabolic engineering · 2017Article
- Article
- Fast growth phenotype of E. coli K-12 from adaptive laboratory evolution does not require intracellular flux rewiring.Metabolic engineering · 2017Article
- Balancing gene expression without library construction via a reusable sRNA pool.Nucleic acids research · 2017Article
- Enzyme I facilitates reverse flux from pyruvate to phosphoenolpyruvate in Escherichia coli.Nature communications · 2017Article
- Comprehensive analysis of glucose and xylose metabolism in Escherichia coli under aerobic and anaerobic conditions byMetabolic engineering · 2017Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Cellular metabolic and regulatory systems are of fundamental interest to biologists and engineers. Incomplete understanding of these complex systems remains an obstacle to progress in biotechnology and metabolic engineering. An established method for obtaining new information on network structure, regulation and dynamics is to study the cellular system following a perturbation such as a genetic knockout. The Keio collection of all viable Escherichia coli single-gene knockouts is facilitating a systematic investigation of the regulation and metabolism of E. coli. Of all omics measurements available, the metabolic flux profile (the fluxome) provides the most direct and relevant representation of the cellular phenotype. Recent advances in (13)C-metabolic flux analysis are now permitting highly precise and accurate flux measurements for investigating cellular systems and guiding metabolic engineering efforts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.