ArticlePlant physiology2014
Dynamics and organization of cortical microtubules as revealed by superresolution structured illumination microscopy.
Article in Plant physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 51 citations in OpenAlex.
- Advanced Imaging for Live-Cell Spatiotemporal Monitoring: Technologies and Applications.Research (Washington, D.C.) · 2026Review
- Technological advances in visualizing and rewiring microtubules during plant development.Journal of experimental botany · 2025Review
- Super-resolution expansion microscopy in plant roots.The Plant cell · 2025Article
- HIV protein Nef expression in human microglia drives the release of distinct Nef-containing extracellular vesicles.Extracellular vesicles and circulating nucleic acids · 2025Article
- Phosphate promotes Arabidopsis root skewing and circumnutation through reorganisation of the microtubule cytoskeleton.The New phytologist · 2024Article
- New insights into the functions and regulations of MAP215/MOR1 and katanin, two conserved microtubule-associated proteins in Arabidopsis.Plant signaling & behavior · 2023Article
- Super-resolution microscopy reveals the number and distribution of topoisomerase IIα and CENH3 molecules within barley metaphase chromosomes.Chromosoma · 2023Article
- At the Nexus between Cytoskeleton and Vacuole: How Plant Cytoskeletons Govern the Dynamics of Large Vacuoles.International journal of molecular sciences · 2023Review
- Fluorescent Fusion Protein Expression in Plant Cells.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Use of red, far-red, and near-infrared light in imaging of yeasts and filamentous fungi.Applied microbiology and biotechnology · 2022Review
- Imaging tools for plant nanobiotechnology.Frontiers in genome editing · 2022Review
- GR24, A Synthetic Strigolactone Analog, and Light Affect the Organization of Cortical Microtubules in Arabidopsis Hypocotyl Cells.Frontiers in plant science · 2021Article
- Imaging flowers: a guide to current microscopy and tomography techniques to study flower development.Journal of experimental botany · 2020Review
- Spatiotemporal Pattern of Ectopic Cell Divisions Contribute to Mis-Shaped Phenotype of Primary and Lateral Roots ofFrontiers in plant science · 2020Article
- Complementary Superresolution Visualization of Composite Plant Microtubule Organization and Dynamics.Frontiers in plant science · 2020Article
- Multicolour three dimensional structured illumination microscopy of immunolabeled plant microtubules and associated proteins.Plant methods · 2019Article
- Variable-angle epifluorescence microscopy characterizes protein dynamics in the vicinity of plasma membrane in plant cells.BMC plant biology · 2018Article
- Advances in Imaging Plant Cell Dynamics.Plant physiology · 2018Review
- Single-molecule fluorescence microscopy review: shedding new light on old problems.Bioscience reports · 2017Review
- Super-resolution Microscopy - Applications in Plant Cell Research.Frontiers in plant science · 2017Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plants employ acentrosomal mechanisms to organize cortical microtubule arrays essential for cell growth and differentiation. Using structured illumination microscopy (SIM) adopted for the optimal documentation of Arabidopsis (Arabidopsis thaliana) hypocotyl epidermal cells, dynamic cortical microtubules labeled with green fluorescent protein fused to the microtubule-binding domain of the mammalian microtubule-associated protein MAP4 and with green fluorescent protein-fused to the alpha tubulin6 were comparatively recorded in wild-type Arabidopsis plants and in the mitogen-activated protein kinase mutant mpk4 possessing the former microtubule marker. The mpk4 mutant exhibits extensive microtubule bundling, due to increased abundance and reduced phosphorylation of the microtubule-associated protein MAP65-1, thus providing a very useful genetic tool to record intrabundle microtubule dynamics at the subdiffraction level. SIM imaging revealed nano-sized defects in microtubule bundling, spatially resolved microtubule branching and release, and finally allowed the quantification of individual microtubules within cortical bundles. Time-lapse SIM imaging allowed the visualization of subdiffraction, short-lived excursions of the microtubule plus end, and dynamic instability behavior of both ends during free, intrabundle, or microtubule-templated microtubule growth and shrinkage. Finally, short, rigid, and nondynamic microtubule bundles in the mpk4 mutant were observed to glide along the parent microtubule in a tip-wise manner. In conclusion, this study demonstrates the potential of SIM for superresolution time-lapse imaging of plant cells, showing unprecedented details accompanying microtubule dynamic organization.
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