ArticleInfection and immunity2014
IsdC from Staphylococcus lugdunensis induces biofilm formation under low-iron growth conditions.
Article in Infection and immunity, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
30 citing papers in PubMed, 44 citations in OpenAlex.
- Comparing clinical manifestations, treatment strategies and outcomes of Staphylococcus lugdunensis bacteraemia versus Staphylococcus aureus bacteraemia - a retrospective cohort study.Internal medicine journal · 2026Article
- Characterization ofBiofilm · 2025Article
- Distribution of Antimicrobial Resistance and Biofilm Production Genes in the Genomic Sequences ofAntibiotics (Basel, Switzerland) · 2025Article
- Article
- Characterization ofAIMS microbiology · 2024Article
- Staphylococcus lugdunensis Uses the Agr Regulatory System to Resist Killing by Host Innate Immune Effectors.Infection and immunity · 2022Article
- Article
- Inactivation of the Response Regulator AgrA Has a Pleiotropic Effect on Biofilm Formation, Pathogenesis and Stress Response in Staphylococcus lugdunensis.Microbiology spectrum · 2022Article
- Staphylococcus lugdunensis: a Skin Commensal with Invasive Pathogenic Potential.Clinical microbiology reviews · 2021Review
- Virulence Factors in Coagulase-Negative Staphylococci.Pathogens (Basel, Switzerland) · 2021Review
- Nanonewton forces betweenNanoscale advances · 2020Article
- Phenotypic and proteomic approaches of the response to iron-limited condition in Staphylococcus lugdunensis.BMC microbiology · 2020Article
- Iron Metabolism at the Interface between Host and Pathogen: From Nutritional Immunity to Antibacterial Development.International journal of molecular sciences · 2020Review
- Role of the LytSR Two-Component Regulatory System inFrontiers in microbiology · 2020Article
- Successful treatment of a unique chronic multi-bacterial scalp infection withAccess microbiology · 2020Article
- Transcriptomic Adjustments ofFrontiers in microbiology · 2019Article
- Article
- Staphylococcus aureus Evasion of Host Immunity in the Setting of Prosthetic Joint Infection: Biofilm and Beyond.Current reviews in musculoskeletal medicine · 2018Review
- Immunoproteomics to identify Staphylococcus aureus antigens expressed in bovine milk during mastitis.Journal of dairy science · 2018Article
- A Novel Antimicrobial Coating Represses Biofilm and Virulence-Related Genes in Methicillin-ResistantFrontiers in microbiology · 2018Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Staphylococcus lugdunensis is a coagulase-negative staphylococcus that is a commensal of humans and an opportunistic pathogen. It can cause a spectrum of infections, including those that are associated with the ability to form biofilm, such as occurs with endocarditis or indwelling medical devices. The genome sequences of two strains revealed the presence of orthologues of the ica genes that are responsible for synthesis of poly-N-acetylglucosamine (PNAG) that is commonly associated with biofilm in other staphylococci. However, we discovered that biofilm formed by a panel of S. lugdunensis isolates growing in iron-restricted medium was susceptible to degradation by proteases and not by metaperiodate, suggesting that the biofilm matrix comprised proteins and not PNAG. When the iron concentration was raised to 1 mM biofilm formation by all strains tested was greatly reduced. A mutant of strain N920143 lacking the entire locus that encodes iron-regulated surface determinant (Isd) proteins was defective in biofilm formation under iron-limited conditions. An IsdC-null mutant was defective, whereas IsdK, IsdJ, and IsdB mutants formed biofilm to the same level as the parental strain. Expression of IsdC was required both for the primary attachment to unconditioned polystyrene and for the accumulation phase of biofilm involving cell-cell interactions. Purified recombinant IsdC protein formed dimers in solution and Lactococcus lactis cells expressing only IsdC adhered to immobilized recombinant IsdC but not to IsdJ, IsdK, or IsdB. This is consistent with a specific homophilic interaction between IsdC molecules on neighboring cells contributing to accumulation of S. lugdunensis biofilm in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.