ArticleJournal of chromatography. A2014
Optimised concentration and purification of retroviruses using membrane chromatography.
Article in Journal of chromatography. A, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed.
- Recent development and application of membrane chromatography.Analytical and bioanalytical chemistry · 2023Review
- Purification of Adeno-Associated Virus (AAV) Serotype 2 fromMembranes · 2022Article
- Rapid and inexpensive purification of adenovirus vectors using an optimised aqueous two-phase technology.Journal of virological methods · 2022Article
- Highly Efficient Purification of Recombinant VSV-∆G-Spike Vaccine against SARS-CoV-2 by Flow-Through Chromatography.Biotech (Basel (Switzerland)) · 2021Article
- A Four-Step Purification Process for Gag VLPs: From Culture Supernatant to High-Purity Lyophilized Particles.Vaccines · 2021Article
- Lentiviral Vector Bioprocessing.Viruses · 2021Review
- Development and Optimization of a Hydrophobic Interaction Chromatography-Based Method of AAV Harvest, Capture, and Recovery.Molecular therapy. Methods & clinical development · 2020Article
- Selection of chromatographic methods for the purification of cell culture-derived Orf virus for its application as a vaccine or viral vector.Journal of biotechnology · 2020Article
- Lentiviral Vector Purification Using Nanofiber Ion-Exchange Chromatography.Molecular therapy. Methods & clinical development · 2019Article
- Probability of viral labeling of neural stem cells in vivo.Neuroscience letters · 2018Article
- Production and purification of high-titer foamy virus vector for the treatment of leukocyte adhesion deficiency.Molecular therapy. Methods & clinical development · 2016Article
- A scalable method to concentrate lentiviral vectors pseudotyped with measles virus glycoproteins.Gene therapy · 2015Article
- Gene Therapy.Advances in biochemical engineering/biotechnologyReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
The ability of an anion exchange membrane to purify a γ-retrovirus was assessed and optimised with respect to different loading and wash buffers. Recoveries of infectious virus greater than 50% were consistently obtained, while specific titre was increased up to one thousand fold when compared to the material loaded. Specific proteins removed and retained by this optimised process were identified by mass spectrometry. It was possible to successfully bind and elute the equivalent of 1.27 × 10(8) Ifu/ml of ion exchange membrane. This could then be highly concentrated, with infectious virus concentrated to a maximum of 420-fold compared to the load.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.