ArticleStructure (London, England : 1993)2014
Identification of an actin binding surface on vinculin that mediates mechanical cell and focal adhesion properties.
Article in Structure (London, England : 1993), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
41 citing papers in PubMed, 62 citations in OpenAlex.
- The bidirectional interplay between RNA processing and mechanotransduction.Cell reports · 2025Review
- Direct Arp2/3-vinculin binding is required for pseudopod extension, but only on compliant substrates and in 3D.iScience · 2025Article
- Afadin mediates cadherin-catenin complex clustering on F-actin linked to cooperative binding and filament curvature.Science advances · 2025Article
- Afadin mediates cadherin-catenin complex clustering on F-actin linked to cooperative binding and filament curvature.bioRxiv : the preprint server for biology · 2024Article
- Split Luciferase Molecular Tension Sensors for Bioluminescent Readout of Mechanical Forces in Biological Systems.ACS sensors · 2024Article
- Detection of fluorescent protein mechanical switching in cellulo.Cell reports methods · 2024Article
- Molecular basis and cellular functions of vinculin-actin directional catch bonding.Nature communications · 2023Article
- Coupling during collective cell migration is controlled by a vinculin mechanochemical switch.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Allosteric activation of vinculin by talin.Nature communications · 2023Article
- Identifying constitutive and context-specific molecular-tension-sensitive protein recruitment within focal adhesions.Developmental cell · 2023Article
- Bottom-up reconstitution of focal adhesion complexes.The FEBS journal · 2022Review
- Analysis of Actin and Focal Adhesion Organisation in U2OS Cells on Polymer Nanostructures.Nanoscale research letters · 2021Article
- Vinculin-mediated axon growth requires interaction with actin but not talin in mouse neocortical neurons.Cellular and molecular life sciences : CMLS · 2021Article
- Adhesion G protein-coupled receptor VLGR1/ADGRV1 regulates cell spreading and migration by mechanosensing at focal adhesions.iScience · 2021Article
- Metavinculin modulates force transduction in cell adhesion sites.Nature communications · 2020Article
- Cx43 and the Actin Cytoskeleton: Novel Roles and Implications for Cell-Cell Junction-Based Barrier Function Regulation.Biomolecules · 2020Review
- Article
- Phosphoinositides regulate force-independent interactions between talin, vinculin, and actin.eLife · 2020Article
- Direct Visualization of Actin Filaments and Actin-Binding Proteins in Neuronal Cells.Frontiers in cell and developmental biology · 2020Review
- Improving Quality, Reproducibility, and Usability of FRET-Based Tension Sensors.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2019Article
Corrections and comments
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Authors and funding
13 authors at 2 institutions in 1 country.
Funding
Abstract
Vinculin, a cytoskeletal scaffold protein essential for embryogenesis and cardiovascular function, localizes to focal adhesions and adherens junctions, connecting cell surface receptors to the actin cytoskeleton. While vinculin interacts with many adhesion proteins, its interaction with filamentous actin regulates cell morphology, motility, and mechanotransduction. Disruption of this interaction lowers cell traction forces and enhances actin flow rates. Although a model for the vinculin:actin complex exists, we recently identified actin-binding deficient mutants of vinculin outside sites predicted to bind actin and developed an alternative model to better define this actin-binding surface, using negative-stain electron microscopy (EM), discrete molecular dynamics, and mutagenesis. Actin-binding deficient vinculin variants expressed in vinculin knockout fibroblasts fail to rescue cell-spreading defects and reduce cellular response to external force. These findings highlight the importance of this actin-binding surface and provide the molecular basis for elucidating additional roles of this interaction, including actin-induced conformational changes that promote actin bundling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.