ArticleEndocrinology2014
Use of a mouse in vitro fertilization model to understand the developmental origins of health and disease hypothesis.
Article in Endocrinology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
57 citing papers in PubMed, 2 syntheses or guidelines pooled it, 132 citations in OpenAlex.
- Biomarker candidates for oocyte and embryo quality assessment in assisted reproduction: A systematic review.JBRA assisted reproduction · 2025Pooled it
- Offspring physiology following the use of IVM, IVF and ICSI: a systematic review and meta-analysis of animal studies.Human reproduction update · 2023Pooled it
- Multi-omics and developmental comparison of direct and conventional warming methods in vitrified human and mouse cleavage-stage embryos.Human reproduction (Oxford, England) · 2026Article
- Preimplantation culture conditions and later-life outcomes: implications for evaluating embryo culture media.The Journal of reproduction and development · 2026Review
- Mouse placentae generated by in vitro fertilization exhibit altered gene expression, activated hypoxia responses, and reduced fitness†.Biology of reproduction · 2026Article
- When less really is more: reducing embryo manipulation to lower metabolic stress and improving long term outcomes.Reproduction, fertility, and development · 2026Review
- Transgenerational Impact of Environmental Change.Advances in experimental medicine and biology · 2026Review
- Redox imbalance in infertility and assisted reproduction: biomarkers, mechanisms, multi-omics integration, and personalised therapeutic strategies.Frontiers in medicine · 2026Review
- Impact of embryo culture and day of embryo transfer on the cardiometabolic health of adult mice†.Biology of reproduction · 2025Article
- Review
- Integrated Multiomics Analyses Reveal Molecular Insights into How Intermittent Fasting Ameliorates Obesity and Increases Fertility in Male Mice.Nutrients · 2025Article
- Bridging the Gap: Animal Models in Next-Generation Reproductive Technologies for Male Fertility Preservation.Life (Basel, Switzerland) · 2023Review
- Metabolomic Profile of Children Conceived With Medically Assisted Technologies.The Journal of clinical endocrinology and metabolism · 2023Article
- Effects of assisted reproductive technology on gene expression in heart and spleen tissues of adult offspring mouse.Frontiers in endocrinology · 2023Article
- Maternal contributions to pregnancy success: from gamete quality to uterine environment.Animal reproduction · 2023Article
- Article
- DNA methylation profile of liver of mice conceived byJournal of developmental origins of health and disease · 2022Article
- Small Noncoding RNAs Contribute to Sperm Oxidative Stress-Induced Programming of Behavioral and Metabolic Phenotypes in Offspring.Oxidative medicine and cellular longevity · 2022Article
- GDF15 negatively regulates RGS16 to impair hepatic lipid metabolism in male mice offspring conceived by in vitro fertilization.American journal of translational research · 2022Article
- Enhanced Synergistic-Antioxidant Activity of Melatonin and Tretinoin by Co-encapsulation into Amphiphilic Chitosan Nanocarriers: During Mice In Vitro Matured Oocyte/Morula-Compact Stage Embryo Culture Model.Reproductive sciences (Thousand Oaks, Calif.) · 2021Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
Abstract
The Developmental Origins of Health and Disease hypothesis holds that alterations to homeostasis during critical periods of development can predispose individuals to adult-onset chronic diseases such as diabetes and metabolic syndrome. It remains controversial whether preimplantation embryo manipulation, clinically used to treat patients with infertility, disturbs homeostasis and affects long-term growth and metabolism. To address this controversy, we have assessed the effects of in vitro fertilization (IVF) on postnatal physiology in mice. We demonstrate that IVF and embryo culture, even under conditions considered optimal for mouse embryo culture, alter postnatal growth trajectory, fat accumulation, and glucose metabolism in adult mice. Unbiased metabolic profiling in serum and microarray analysis of pancreatic islets and insulin sensitive tissues (liver, skeletal muscle, and adipose tissue) revealed broad changes in metabolic homeostasis, characterized by systemic oxidative stress and mitochondrial dysfunction. Adopting a candidate approach, we identify thioredoxin-interacting protein (TXNIP), a key molecule involved in integrating cellular nutritional and oxidative states with metabolic response, as a marker for preimplantation stress and demonstrate tissue-specific epigenetic and transcriptional TXNIP misregulation in selected adult tissues. Importantly, dysregulation of TXNIP expression is associated with enrichment for H4 acetylation at the Txnip promoter that persists from the blastocyst stage through adulthood in adipose tissue. Our data support the vulnerability of preimplantation embryos to environmental disturbance and demonstrate that conception by IVF can reprogram metabolic homeostasis through metabolic, transcriptional, and epigenetic mechanisms with lasting effects for adult growth and fitness. This study has wide clinical relevance and underscores the importance of continued follow-up of IVF-conceived offspring.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.