ArticleNucleic acids research2014
A eukaryotic-like 3' untranslated region in Salmonella enterica hilD mRNA.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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34 citing papers in PubMed, 54 citations in OpenAlex.
- A Small RNA Derived From the 5' End of the IS200 tnpA Transcript Regulates Multiple Virulence Regulons in Salmonella typhimurium.Molecular microbiology · 2025Article
- "A high throughput flow cytometry assay for quantifying type 3 secretion system assembly inbioRxiv : the preprint server for biology · 2025Article
- Genomic epidemiology and phenotypic characterisation of Salmonella enterica serovar Panama in Victoria, Australia.PLoS neglected tropical diseases · 2024Article
- Construction of a constitutively active type III secretion system for heterologous protein secretion.Applied microbiology and biotechnology · 2023Article
- Small RNAs Activate Salmonella Pathogenicity Island 1 by Modulating mRNA Stability through theJournal of bacteriology · 2023Article
- Identifying Bacterial Lineages in Salmonella by Flow Cytometry.EcoSal Plus · 2022Review
- Pervasive transcription enhances the accessibility of H-NS-silenced promoters and generates bistability inProceedings of the National Academy of Sciences of the United States of America · 2022Article
- RNase III-CLASH of multi-drug resistant Staphylococcus aureus reveals a regulatory mRNA 3'UTR required for intermediate vancomycin resistance.Nature communications · 2022Article
- Article
- Modulation of AggR levels reveals features of virulence regulation in enteroaggregative E. coli.Communications biology · 2021Article
- Long-Distance Effects of H-NS Binding in the Control ofJournal of bacteriology · 2021Article
- Regulatory protein HilD stimulates Salmonella Typhimurium invasiveness by promoting smooth swimming via the methyl-accepting chemotaxis protein McpC.Nature communications · 2021Article
- Differential evolution in 3'UTRs leads to specific gene expression in Staphylococcus.Nucleic acids research · 2020Article
- Bacterial 3'UTRs: A Useful Resource in Post-transcriptional Regulation.Frontiers in molecular biosciences · 2020Review
- The Rho-Independent Transcription Terminator for theInfection and immunity · 2019Article
- The Small RNA PinT Contributes to PhoP-Mediated Regulation of theJournal of bacteriology · 2019Article
- Oxygen-dependent regulation of SPI1 type three secretion system by small RNAs in Salmonella enterica serovar Typhimurium.Molecular microbiology · 2019Article
- 3'untranslated regions: regulation at the end of the road.Current genetics · 2019Review
- Contribution of SPI-1 bistability to Salmonella enterica cooperative virulence: insights from single cell analysis.Scientific reports · 2018Article
- Regulatory RNAs in Virulence and Host-Microbe Interactions.Microbiology spectrum · 2018Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Long 3' untranslated regions (3'UTRs) are common in eukaryotic mRNAs. In contrast, long 3'UTRs are rare in bacteria, and have not been characterized in detail. We describe a 3'UTR of 310 nucleotides in hilD mRNA, a transcript that encodes a transcriptional activator of Salmonella enterica pathogenicity island 1 (SPI-1). Deletion of the hilD 3'UTR increases the hilD mRNA level, suggesting that the hilD 3'UTR may play a role in hilD mRNA turnover. Cloning of the hilD 3'UTR downstream of the green fluorescent protein (gfp) gene decreases green fluorescent protein (GFP) activity in both Escherichia coli and S. enterica, indicating that the hilD 3'UTR can act as an independent module. S. enterica mutants lacking either ribonuclease E or polynucleotide phosphorylase contain similar amounts of hilD and hilD Δ3'UTR mRNAs, suggesting that the hilD 3'UTR is a target for hilD mRNA degradation by the degradosome. The hilD 3'UTR is also necessary for modulation of hilD and SPI-1 expression by the RNA chaperone Hfq. Overexpression of SPI-1 in the absence of the hilD 3'UTR retards Salmonella growth and causes uncontrolled invasion of epithelial cells. Based on these observations, we propose that the S. enterica hilD 3'UTR is a cis-acting element that contributes to cellular homeostasis by promoting hilD mRNA turnover.
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