ArticleJournal of bacteriology2014
Function of the SpoVAEa and SpoVAF proteins of Bacillus subtilis spores.
Article in Journal of bacteriology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 40 citations in OpenAlex.
- A mathematical kinetic model of memory iniScience · 2026Article
- Recent progress in proteins regulating the germination ofJournal of bacteriology · 2025Review
- Newly identified SpoVAF/FigP complex: the role inApplied and environmental microbiology · 2025Article
- Identification and characterization of theJournal of bacteriology · 2024Article
- SpoVAF and FigP assemble into oligomeric ion channels that enhance spore germination.Genes & development · 2024Article
- Electrochemical potential enables dormant spores to integrate environmental signals.Science (New York, N.Y.) · 2022Article
- Visualization of SpoVAEa Protein Dynamics in Dormant Spores ofMicrobiology spectrum · 2022Article
- The SpoVA membrane complex is required for dipicolinic acid import during sporulation and export during germination.Genes & development · 2022Article
- Organization and dynamics of the SpoVAEa protein and its surrounding inner membrane lipids, upon germination of Bacillus subtilis spores.Scientific reports · 2022Article
- Genetic Evidence for Signal Transduction within the Bacillus subtilis GerA Germinant Receptor.Journal of bacteriology · 2022Article
- Clostridioides difficile spore germination: initiation to DPA release.Current opinion in microbiology · 2022Review
- Dynamics of Germinosome Formation and FRET-Based Analysis of Interactions between GerD and Germinant Receptor Subunits inInternational journal of molecular sciences · 2021Article
- Clostridioides difficile SpoVAD and SpoVAE Interact and Are Required for Dipicolinic Acid Uptake into Spores.Journal of bacteriology · 2021Article
- Suboptimal Bacillus licheniformis and Bacillus weihenstephanensis Spore Incubation Conditions Increase Heterogeneity of Spore Outgrowth Time.Applied and environmental microbiology · 2020Article
- The requirement for co-germinants during Clostridium difficile spore germination is influenced by mutations in yabG and cspA.PLoS pathogens · 2019Article
- Identification of L-Valine-initiated-germination-active genes in Bacillus subtilis using Tn-seq.PloS one · 2019Article
- Analyzing AbrB-Knockout Effects through Genome and Transcriptome Sequencing ofFrontiers in microbiology · 2018Article
- Spore Heat Activation Requirements and Germination Responses Correlate with Sequences of Germinant Receptors and with the Presence of a SpecificApplied and environmental microbiology · 2017Article
- Genetic Characterization of the Exceptionally High Heat Resistance of the Non-toxic SurrogateFrontiers in microbiology · 2017Article
- Characterization of Clostridium difficile Spores Lacking Either SpoVAC or Dipicolinic Acid Synthetase.Journal of bacteriology · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Bacillus subtilis spoVAEa and spoVAF genes are expressed in developing spores as members of the spoVA operon, which encodes proteins essential for the uptake and release of dipicolinic acid (DPA) during spore formation and germination. SpoVAF is likely an integral inner spore membrane protein and exhibits sequence identity to A subunits of the spore's nutrient germinant receptors (GRs), while SpoVAEa is a soluble protein with no obvious signals to allow its passage across a membrane. However, like SpoVAD, SpoVAEa is present on the outer surface of the spore's inner membrane, as SpoVAEa was accessible to an external biotinylation agent in spores and SpoVAEa disappeared in parallel with SpoVAD during proteinase K treatment of germinated spores. SpoVAEa and SpoVAD were also distributed similarly in fractions of disrupted dormant spores. Unlike spoVAD, spoVAEa is absent from the genomes of some spore-forming members of the Bacillales and Clostridiales orders, although SpoVAEa's amino acid sequence is conserved in species containing spoVAEa. B. subtilis strains lacking SpoVAF or SpoVAEa and SpoVAF sporulated normally, and the spores had normal DPA levels. Spores lacking SpoVAF or SpoVAEa and SpoVAF also germinated normally with non-GR-dependent germinants but more slowly than wild-type spores with GR-dependent germinants, and this germination defect was complemented by ectopic expression of the missing proteins.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.