ArticleApplied and environmental microbiology2014
Nuclear localization of Haa1, which is linked to its phosphorylation status, mediates lactic acid tolerance in Saccharomyces cerevisiae.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 37 citations in OpenAlex.
- Advances and perspectives in genetically encoded biosensors for biomarker detection and theranostic circuits.Synthetic and systems biotechnology · 2027Review
- Dual regulatory mechanisms of YGP1 expression in response to glucose availability in Saccharomyces cerevisiae.PloS one · 2026Article
- Regulatory mechanism of Haa1p and Hap4p in Saccharomyces cerevisiae to mixed acetic acid and formic acid when fermenting mixed glucose and xylose.Microbial cell factories · 2025Article
- Phosphate-dependent nuclear export via a non-classical NES class recognized by exportin Msn5.Nature communications · 2025Article
- The transcription factor CgHaa1 plays a role in virulence of the pathogenic yeast Candida glabrata.FEMS yeast research · 2025Article
- Transcriptional response of Saccharomyces cerevisiae to lactic acid enantiomers.Applied microbiology and biotechnology · 2024Article
- Protein Kinase A Negatively Regulates the Acetic Acid Stress Response inMicroorganisms · 2024Article
- General mechanisms of weak acid-tolerance and current strategies for the development of tolerant yeasts.World journal of microbiology & biotechnology · 2023Review
- Engineering Flocculation for Improved Tolerance and Production of d-Lactic Acid inJournal of fungi (Basel, Switzerland) · 2023Article
- Unravel the regulatory mechanism of Yrr1p phosphorylation in response to vanillin stress in Saccharomyces cerevisiae.Microbial cell factories · 2023Article
- Metabolic Engineering and Adaptive Evolution for Efficient Production of l-Lactic Acid in Saccharomyces cerevisiae.Microbiology spectrum · 2022Article
- Identification of acetic acid sensitive strains through biosensor-based screening of a Saccharomyces cerevisiae CRISPRi library.Microbial cell factories · 2022Article
- Response mechanisms of Saccharomyces cerevisiae to the stress factors present in lignocellulose hydrolysate and strategies for constructing robust strains.Biotechnology for biofuels and bioproducts · 2022Review
- The cell wall and the response and tolerance to stresses of biotechnological relevance in yeasts.Frontiers in microbiology · 2022Review
- Development of an Haa1-based biosensor for acetic acid sensing in Saccharomyces cerevisiae.FEMS yeast research · 2021Article
- Mechanisms underlying lactic acid tolerance and its influence on lactic acid production inMicrobial cell (Graz, Austria) · 2021Review
- Improvement of lactic acid tolerance by cocktail δ-integration strategy and identification of the transcription factor PDR3 responsible for lactic acid tolerance in yeast Saccharomyces cerevisiae.World journal of microbiology & biotechnology · 2021Article
- Carbon Catabolite Repression in Yeast is Not Limited to Glucose.Scientific reports · 2019Article
- Karyopherin Msn5 is involved in a novel mechanism controlling the cellular level of cell cycle regulators Cln2 and Swi5.Cell cycle (Georgetown, Tex.) · 2019Article
- Activation of Haa1 and War1 transcription factors by differential binding of weak acid anions in Saccharomyces cerevisiae.Nucleic acids research · 2019Article
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Authors and funding
6 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Improvement of the lactic acid resistance of the yeast Saccharomyces cerevisiae is important for the application of the yeast in industrial production of lactic acid from renewable resources. However, we still do not know the precise mechanisms of the lactic acid adaptation response in yeast and, consequently, lack effective approaches for improving its lactic acid tolerance. To enhance our understanding of the adaptation response, we screened for S. cerevisiae genes that confer enhanced lactic acid resistance when present in multiple copies and identified the transcriptional factor Haa1 as conferring resistance to toxic levels of lactic acid when overexpressed. The enhanced tolerance probably results from increased expression of its target genes. When cells that expressed Haa1 only from the endogenous promoter were exposed to lactic acid stress, the main subcellular localization of Haa1 changed from the cytoplasm to the nucleus within 5 min. This nuclear accumulation induced upregulation of the Haa1 target genes YGP1, GPG1, and SPI1, while the degree of Haa1 phosphorylation observed under lactic acid-free conditions decreased. Disruption of the exportin gene MSN5 led to accumulation of Haa1 in the nucleus even when no lactic acid was present. Since Msn5 was reported to interact with Haa1 and preferentially exports phosphorylated cargo proteins, our results suggest that regulation of the subcellular localization of Haa1, together with alteration of its phosphorylation status, mediates the adaptation to lactic acid stress in yeast.
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