ArticleGenome biology and evolution2014
A nonmitochondrial hydrogen production in Naegleria gruberi.
Article in Genome biology and evolution, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 29 citations in OpenAlex.
- Article
- Long-term survive of Aliarcobacter butzleri in two models symbiotic interaction with Acanthamoeba castellanii.Archives of microbiology · 2022Article
- Localization and functional characterization of the alternative oxidase in Naegleria.The Journal of eukaryotic microbiology · 2022Article
- Copper Metabolism inFrontiers in cell and developmental biology · 2022Article
- Multiple integrated metabolic strategies allow foraminiferan protists to thrive in anoxic marine sediments.Science advances · 2021Article
- Evolutionary History of Mitochondrial Genomes in Discoba, Including the Extreme Halophile Pleurostomum flabellatum (Heterolobosea).Genome biology and evolution · 2021Article
- Adaptive iron utilization compensates for the lack of an inducible uptake system in Naegleria fowleri and represents a potential target for therapeutic intervention.PLoS neglected tropical diseases · 2020Article
- Insight into the Lifestyle of AmoebaMicroorganisms · 2020Article
- Molecular Hydrogen Metabolism: a Widespread Trait of Pathogenic Bacteria and Protists.Microbiology and molecular biology reviews : MMBR · 2020Review
- Branched late-steps of the cytosolic iron-sulphur cluster assembly machinery of Trypanosoma brucei.PLoS pathogens · 2018Article
- Identification and characterisation of a cryptic Golgi complex inJournal of cell science · 2018Article
- Diversity and origins of anaerobic metabolism in mitochondria and related organelles.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2015Review
- Biohydrogen production: strategies to improve process efficiency through microbial routes.International journal of molecular sciences · 2015Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Naegleria gruberi is a free-living heterotrophic aerobic amoeba well known for its ability to transform from an amoeba to a flagellate form. The genome of N. gruberi has been recently published, and in silico predictions demonstrated that Naegleria has the capacity for both aerobic respiration and anaerobic biochemistry to produce molecular hydrogen in its mitochondria. This finding was considered to have fundamental implications on the evolution of mitochondrial metabolism and of the last eukaryotic common ancestor. However, no actual experimental data have been shown to support this hypothesis. For this reason, we have decided to investigate the anaerobic metabolism of the mitochondrion of N. gruberi. Using in vivo biochemical assays, we have demonstrated that N. gruberi has indeed a functional [FeFe]-hydrogenase, an enzyme that is attributed to anaerobic organisms. Surprisingly, in contrast to the published predictions, we have demonstrated that hydrogenase is localized exclusively in the cytosol, while no hydrogenase activity was associated with mitochondria of the organism. In addition, cytosolic localization displayed for HydE, a marker component of hydrogenase maturases. Naegleria gruberi, an obligate aerobic organism and one of the earliest eukaryotes, is producing hydrogen, a function that raises questions on the purpose of this pathway for the lifestyle of the organism and potentially on the evolution of eukaryotes.
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