ArticleRNA (New York, N.Y.)2014
The β-actin mRNA zipcode regulates epithelial adherens junction assembly but not maintenance.
Article in RNA (New York, N.Y.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 19 citations in OpenAlex.
- Cytoplasmic localization of the mRNA encoding actin regulator, Serendipity-bioRxiv : the preprint server for biology · 2025Article
- Examination of the role of the rumen in zinc metabolism.Journal of animal science · 2025Article
- Unique and redundant functions of cytoplasmic actins and nonmuscle myosin II isoforms at epithelial junctions.Annals of the New York Academy of Sciences · 2022Review
- Cytoplasmic pressure maintains epithelial integrity and inhibits cell motility.Physical biology · 2021Article
- RNA-biology ruling cancer progression? Focus on 3'UTRs and splicing.Cancer metastasis reviews · 2020Review
- Loss of β-Cytoplasmic Actin in the Intestinal Epithelium Increases Gut Barrier PermeabilityFrontiers in cell and developmental biology · 2020Article
- Posttranscriptional and Posttranslational Regulation of Actin.Anatomical record (Hoboken, N.J. : 2007) · 2018Review
- Close encounters of the RNAi kind: the silencing life of the adherens junctions.Current opinion in cell biology · 2018Review
- RNA localization regulates diverse and dynamic cellular processes.Traffic (Copenhagen, Denmark) · 2018Review
- The makings of the 'actin code': regulation of actin's biological function at the amino acid and nucleotide level.Journal of cell science · 2018Review
- Cadherin complexes recruit mRNAs and RISC to regulate epithelial cell signaling.The Journal of cell biology · 2017Article
- Quantifying cadherin mechanotransduction machinery assembly/disassembly dynamics using fluorescence covariance analysis.Scientific reports · 2016Article
- Balancing spatially regulated β-actin translation and dynamin-mediated endocytosis is required to assemble functional epithelial monolayers.Cytoskeleton (Hoboken, N.J.) · 2015Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Epithelial cell-cell contact stimulates actin cytoskeleton remodeling to down-regulate branched filament polymerization-driven lamellar protrusion and subsequently to assemble linear actin filaments required for E-cadherin anchoring during adherens junction complex assembly. In this manuscript, we demonstrate that de novo protein synthesis, the β-actin 3' UTR, and the β-actin mRNA zipcode are required for epithelial adherens junction complex assembly but not maintenance. Specifically, we demonstrate that perturbing cell-cell contact-localized β-actin monomer synthesis causes epithelial adherens junction assembly defects. Consequently, inhibiting β-actin mRNA zipcode/ZBP1 interactions with β-actin mRNA zipcode antisense oligonucleotides, to intentionally delocalize β-actin monomer synthesis, is sufficient to perturb adherens junction assembly following epithelial cell-cell contact. Additionally, we demonstrate active RhoA, the signal required to drive zipcode-mediated β-actin mRNA targeting, is localized at epithelial cell-cell contact sites in a β-actin mRNA zipcode-dependent manner. Moreover, chemically inhibiting Src kinase activity prevents the local stimulation of β-actin monomer synthesis at cell-cell contact sites while inhibiting epithelial adherens junction assembly. Together, these data demonstrate that epithelial cell-cell contact stimulates β-actin mRNA zipcode-mediated monomer synthesis to spatially regulate actin filament remodeling, thereby controlling adherens junction assembly to modulate cell and tissue adhesion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.