ArticlePloS one2014
Δ12-Fatty acid desaturase from Candida parapsilosis is a multifunctional desaturase producing a range of polyunsaturated and hydroxylated fatty acids.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 26 citations in OpenAlex.
- Genomic Insights into Lipid Dependency in Atypical Strains of Malassezia pachydermatis.Mycopathologia · 2025Article
- Inhibition of cell cycle-dependent hyphal and biofilm formation by a novel cytochalasin 19,20‑epoxycytochalasin Q in Candida albicans.Scientific reports · 2023Article
- Comparative Analysis of TwoCells · 2023Article
- Lipid metabolism of the oleaginous yeast Lipomyces starkeyi.Applied microbiology and biotechnology · 2020Review
- Desaturase specificity is controlled by the physicochemical properties of a single amino acid residue in the substrate binding tunnel.Computational and structural biotechnology journal · 2020Article
- Analysis ofFrontiers in cellular and infection microbiology · 2020Article
- Physiological and Transcriptional Responses of Candida parapsilosis to Exogenous Tyrosol.Applied and environmental microbiology · 2019Article
- Candida parapsilosis: from Genes to the Bedside.Clinical microbiology reviews · 2019Review
- An Evolutionary Perspective on Linoleic Acid Synthesis in Animals.Evolutionary biology · 2018Article
- Article
- Three, two, one yeast fatty acid desaturases: regulation and function.World journal of microbiology & biotechnology · 2017Review
- Evolution of moth sex pheromone composition by a single amino acid substitution in a fatty acid desaturase.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
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5 authors at 2 institutions in 1 country.
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Abstract
Numerous Δ12-, Δ15- and multifunctional membrane fatty acid desaturases (FADs) have been identified in fungi, revealing great variability in the enzymatic specificities of FADs involved in biosynthesis of polyunsaturated fatty acids (PUFAs). Here, we report gene isolation and characterization of novel Δ12/Δ15- and Δ15-FADs named CpFad2 and CpFad3, respectively, from the opportunistic pathogenic yeast Candida parapsilosis. Overexpression of CpFad3 in Saccharomyces cerevisiae strains supplemented with linoleic acid (Δ9,Δ12-18:2) and hexadecadienoic acid (Δ9,Δ12-16:2) leads to accumulation of Δ15-PUFAs, i.e., α-linolenic acid (Δ9,Δ12,Δ15-18:3) and hexadecatrienoic acid with an unusual terminal double bond (Δ9,Δ12,Δ15-16:3). CpFad2 produces a range of Δ12- and Δ15-PUFAs. The major products of CpFad2 are linoleic and hexadecadienoic acid (Δ9,Δ12-16:2), accompanied by α-linolenic acid and hexadecatrienoic acid (Δ9,Δ12,Δ15-16:3). Using GC/MS analysis of trimethylsilyl derivatives, we identified ricinoleic acid (12-hydroxy-9-octadecenoic acid) as an additional product of CpFad2. These results demonstrate that CpFAD2 is a multifunctional FAD and indicate that detailed analysis of fatty acid derivatives might uncover a range of enzymatic selectivities in other Δ12-FADs from budding yeasts (Ascomycota: Saccharomycotina).
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