ArticleJournal of cell science2014
GEF-H1 functions in apical constriction and cell intercalations and is essential for vertebrate neural tube closure.
Article in Journal of cell science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
35 citing papers in PubMed, 50 citations in OpenAlex.
- Myocardin-related transcription factor regulates actomyosin contractility and apical junction remodeling during vertebrate neural tube closure.Development (Cambridge, England) · 2025Article
- The Mechanics of Building Functional Organs.Cold Spring Harbor perspectives in biology · 2025Review
- Linking planar polarity signalling to actomyosin contractility during vertebrate neurulation.Open biology · 2024Article
- The RhoGEF protein Plekhg5 self-associates via its PH domain to regulate apical cell constriction.Molecular biology of the cell · 2024Article
- Change in RhoGAP and RhoGEF availability drives transitions in cortical patterning and excitability in Drosophila.Current biology : CB · 2024Article
- Change in RhoGAP and RhoGEF availability drives transitions in cortical patterning and excitability inbioRxiv : the preprint server for biology · 2023Article
- The RhoGEF protein Plekhg5 regulates medioapical and junctional actomyosin dynamics of apical constriction duringMolecular biology of the cell · 2023Article
- Pathogenesis of neural tube defects: The regulation and disruption of cellular processes underlying neural tube closure.WIREs mechanisms of disease · 2022Review
- Lmo7 recruits myosin II heavy chain to regulate actomyosin contractility and apical domain size in Xenopus ectoderm.Development (Cambridge, England) · 2022Article
- Force-dependent remodeling of cytoplasmic ZO-1 condensates contributes to cell-cell adhesion through enhancing tight junctions.iScience · 2022Article
- Assays for Apical Constriction Using the Xenopus Model.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Hallmarks of primary neurulation are conserved in the zebrafish forebrain.Communications biology · 2021Article
- Sonic hedgehog signaling directs patterned cell remodeling during cranial neural tube closure.eLife · 2020Article
- Article
- MASTL promotes cell contractility and motility through kinase-independent signaling.The Journal of cell biology · 2020Article
- Apical polarity proteins recruit the RhoGEF Cysts to promote junctional myosin assembly.The Journal of cell biology · 2019Article
- Expansion and contraction of the umbrella cell apical junctional ring in response to bladder filling and voiding.Molecular biology of the cell · 2019Article
- Anillin regulates epithelial cell mechanics by structuring the medial-apical actomyosin network.eLife · 2019Article
- The RhoGEF protein Plekhg5 regulates apical constriction of bottle cells during gastrulation.Development (Cambridge, England) · 2018Article
- Esrp1-Regulated Splicing of Arhgef11 Isoforms Is Required for Epithelial Tight Junction Integrity.Cell reports · 2018Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Rho family GTPases regulate many morphogenetic processes during vertebrate development including neural tube closure. Here we report a function for GEF-H1/Lfc/ArhGEF2, a RhoA-specific guanine nucleotide exchange factor that functions in neurulation in Xenopus embryos. Morpholino-mediated depletion of GEF-H1 resulted in severe neural tube defects, which were rescued by GEF-H1 RNA. Lineage tracing of GEF-H1 morphants at different developmental stages revealed abnormal cell intercalation and apical constriction, suggesting that GEF-H1 regulates these cell behaviors. Molecular marker analysis documented defects in myosin II light chain (MLC) phosphorylation, Rab11 and F-actin accumulation in GEF-H1-depleted cells. In gain-of-function studies, overexpressed GEF-H1 induced Rho-associated kinase-dependent ectopic apical constriction - marked by apical accumulation of phosphorylated MLC, γ-tubulin and F-actin in superficial ectoderm - and stimulated apical protrusive activity of deep ectoderm cells. Taken together, our observations newly identify functions of GEF-H1 in morphogenetic movements that lead to neural tube closure.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.