ArticleNature methods2014
Quantifying Drosophila food intake: comparative analysis of current methodology.
Article in Nature methods, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 127 papers.
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Who cites it
127 citing papers in PubMed, 237 citations in OpenAlex.
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- Structure, function, and quantitative biology of the Drosophila gut microbiome.Current opinion in microbiology · 2025Review
- High-throughput measurement of Drosophila feeding behavior.Cell reports methods · 2025Article
- Downregulation of Pten Improves Huntington's Disease Phenotype by Reducing Htt Aggregates and Cell Death.Molecular neurobiology · 2025Article
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- Exploration-exploitation trade-off is regulated by metabolic state and taste value inbioRxiv : the preprint server for biology · 2024Article
- Quantifying Food Intake in Caenorhabditis elegans by Measuring Bacterial Clearance.Journal of visualized experiments : JoVE · 2024Article
67 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Food intake is a fundamental parameter in animal studies. Despite the prevalent use of Drosophila in laboratory research, precise measurements of food intake remain challenging in this model organism. Here, we compare several common Drosophila feeding assays: the capillary feeder (CAFE), food labeling with a radioactive tracer or colorimetric dye and observations of proboscis extension (PE). We show that the CAFE and radioisotope labeling provide the most consistent results, have the highest sensitivity and can resolve differences in feeding that dye labeling and PE fail to distinguish. We conclude that performing the radiolabeling and CAFE assays in parallel is currently the best approach for quantifying Drosophila food intake. Understanding the strengths and limitations of methods for measuring food intake will greatly advance Drosophila studies of nutrition, behavior and disease.
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