ArticleNature methods2014
Transcriptional profiling of cells sorted by RNA abundance.
Article in Nature methods, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The trial behind it
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Who cites it
23 citing papers in PubMed.
- Injury polarized CD4Redox biology · 2026Article
- Unveiling heterogeneity in rare cells by combining RNA-based sorting and scRNA-seq.Nature genetics · 2025Article
- Comparison of bias and resolvability in single-cell and single-transcript methods.Communications biology · 2021Article
- Microdroplet-based one-step RT-PCR for ultrahigh throughput single-cell multiplex gene expression analysis and rare cell detection.Scientific reports · 2021Article
- Article
- Single-cell transcriptional landscapes reveal HIV-1-driven aberrant host gene transcription as a potential therapeutic target.Science translational medicine · 2020Article
- FIN-Seq: transcriptional profiling of specific cell types from frozen archived tissue of the human central nervous system.Nucleic acids research · 2020Article
- Article
- ClampFISH detects individual nucleic acid molecules using click chemistry-based amplification.Nature biotechnology · 2018Article
- Cholesterol signaling in single cells: lessons from STAR and sm-FISH.Journal of molecular endocrinology · 2018Review
- Systematic identification of factors mediating accelerated mRNA degradation in response to changes in environmental nitrogen.PLoS genetics · 2018Article
- Isolation of intact RNA from murine CD4Journal of immunological methods · 2018Article
- Transcriptome Analysis of Mycobacteria-Specific CD4Journal of immunology (Baltimore, Md. : 1950) · 2017Article
- Cerebral cortical neuron diversity and development at single-cell resolution.Current opinion in neurobiology · 2017Review
- RNA-Seq following PCR-based sorting reveals rare cell transcriptional signatures.BMC genomics · 2016Article
- Simultaneous, Single-Cell Measurement of Messenger RNA, Cell Surface Proteins, and Intracellular Proteins.Current protocols in cytometry · 2016Article
- Fixed single-cell transcriptomic characterization of human radial glial diversity.Nature methods · 2016Article
- A microfluidic approach to parallelized transcriptional profiling of single cells.Microfluidics and nanofluidics · 2015Article
- Functional footprinting of regulatory DNA.Nature methods · 2015Article
- Variation in transcriptome size: are we getting the message?Chromosoma · 2015Review
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
We have developed a quantitative technique for sorting cells on the basis of endogenous RNA abundance, with a molecular resolution of 10-20 transcripts. We demonstrate efficient and unbiased RNA extraction from transcriptionally sorted cells and report a high-fidelity transcriptome measurement of mouse induced pluripotent stem cells (iPSCs) isolated from a heterogeneous reprogramming culture. This method is broadly applicable to profiling transcriptionally distinct cellular states without requiring antibodies or transgenic fluorescent proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.