ArticleEukaryotic cell2014
Depletion of the Trypanosome Pumilio domain protein PUF2 or of some other essential proteins causes transcriptome changes related to coding region length.
Article in Eukaryotic cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 28 citations in OpenAlex.
- Decoding post-transcriptional gene expression controls in trypanosomatids using machine learning.Wellcome open research · 2025Article
- A unique mRNA decapping complex in trypanosomes.Nucleic acids research · 2023Article
- Identification of Genes Associated with the Impairment of Olfactory and Gustatory Functions in COVID-19 via Machine-Learning Methods.Life (Basel, Switzerland) · 2023Article
- Interactions of theParasitology · 2022Article
- Insights into the functions and RNA binding ofParasitology · 2021Article
- Roles of the Pumilio domain protein PUF3 inParasitology · 2020Article
- In Vivo Tethering System to Isolate RNA-Binding Proteins Regulating mRNA Decay in Leishmania.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Regulation of gene expression in trypanosomatids: living with polycistronic transcription.Open biology · 2019Article
- Article
- Transcriptomes of Trypanosoma brucei rhodesiense from sleeping sickness patients, rodents and culture: Effects of strain, growth conditions and RNA preparation methods.PLoS neglected tropical diseases · 2018Article
- The Pumilio-domain protein PUF6 contributes to SIDER2 retroposon-mediated mRNA decay inRNA (New York, N.Y.) · 2017Article
- Article
- Article
- Transcriptomes of newly-isolated Trypanosoma brucei rhodesiense reveal hundreds of mRNAs that are co-regulated with stumpy-form markers.BMC genomics · 2015Article
- NMD3 regulates both mRNA and rRNA nuclear export in African trypanosomes via an XPOI-linked pathway.Nucleic acids research · 2015Article
- Trypanosoma brucei RRM1 is a nuclear RNA-binding protein and modulator of chromatin structure.mBio · 2015Article
- Polysomes of Trypanosoma brucei: Association with Initiation Factors and RNA-Binding Proteins.PloS one · 2015Article
- Transcriptome-wide analysis of trypanosome mRNA decay reveals complex degradation kinetics and suggests a role for co-transcriptional degradation in determining mRNA levels.Molecular microbiology · 2014Article
- PUF Proteins as Critical RNA-Binding Proteins in TriTryp Parasites: A Review Article.Iranian journal of parasitologyReview
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pumilio domain RNA-binding proteins are known mainly as posttranscriptional repressors of gene expression that reduce mRNA translation and stability. Trypanosoma brucei has 11 PUF proteins. We show here that PUF2 is in the cytosol, with roughly the same number of molecules per cell as there are mRNAs. Although PUF2 exhibits a low level of in vivo RNA binding, it is not associated with polysomes. PUF2 also decreased reporter mRNA levels in a tethering assay, consistent with a repressive role. Depletion of PUF2 inhibited growth of bloodstream-form trypanosomes, causing selective loss of mRNAs with long open reading frames and increases in mRNAs with shorter open reading frames. Reexamination of published RNASeq data revealed the same trend in cells depleted of some other proteins. We speculate that these length effects could be caused by inhibition of the elongation phase of transcription or by an influence of translation status or polysomal conformation on mRNA decay.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.