ArticleAnesthesiology2014
Inhibition of the ubiquitin-proteasome pathway does not protect against ventilator-induced accelerated proteolysis or atrophy in the diaphragm.
Article in Anesthesiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 38 citations in OpenAlex.
- Ventilator-induced diaphragmatic dysfunction: pathophysiology, monitoring and advances in potential treatment and prevention.European respiratory review : an official journal of the European Respiratory Society · 2025Review
- Diaphragm Function in Health and Disease.Advances in experimental medicine and biology · 2025Review
- Review
- Research progress on the pathogenesis and treatment of ventilator-induced diaphragm dysfunction.Heliyon · 2023Review
- Small-Molecule Inhibition of MuRF1 Prevents Early Disuse-Induced Diaphragmatic Dysfunction and Atrophy.International journal of molecular sciences · 2023Article
- Intensive Care Unit Acquired Weakness Is Associated with Rapid Changes to Skeletal Muscle Proteostasis.Cells · 2022Article
- Article
- Acute liver injury following acetaminophen administration does not activate atrophic pathways in the mouse diaphragm.Scientific reports · 2021Article
- Article
- Redox Control of Proteolysis During Inactivity-Induced Skeletal Muscle Atrophy.Antioxidants & redox signaling · 2020Review
- Emerging Strategies Targeting Catabolic Muscle Stress Relief.International journal of molecular sciences · 2020Review
- Disturbances in Calcium Homeostasis Promotes Skeletal Muscle Atrophy: Lessons From Ventilator-Induced Diaphragm Wasting.Frontiers in physiology · 2020Review
- Small-molecule-mediated chemical knock-down of MuRF1/MuRF2 and attenuation of diaphragm dysfunction in chronic heart failure.Journal of cachexia, sarcopenia and muscle · 2019Article
- Effects of exercise preconditioning and HSP72 on diaphragm muscle function during mechanical ventilation.Journal of cachexia, sarcopenia and muscle · 2019Article
- Endurance exercise protects skeletal muscle against both doxorubicin-induced and inactivity-induced muscle wasting.Pflugers Archiv : European journal of physiology · 2019Review
- [Diaphragm dysfunction : Facts for clinicians].Medizinische Klinik, Intensivmedizin und Notfallmedizin · 2018Review
- The Renin-Angiotensin System and Skeletal Muscle.Exercise and sport sciences reviews · 2018Review
- Exercise prevents impaired autophagy and proteostasis in a model of neurogenic myopathy.Scientific reports · 2018Article
- Crosstalk between autophagy and oxidative stress regulates proteolysis in the diaphragm during mechanical ventilation.Free radical biology & medicine · 2018Article
- The role of calpains in ventilator-induced diaphragm atrophy.Intensive care medicine experimental · 2017Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundMechanical ventilation (MV) is a life-saving intervention in patients with acute respiratory failure. However, prolonged MV results in ventilator-induced diaphragm dysfunction (VIDD), a condition characterized by both diaphragm fiber atrophy and contractile dysfunction. Previous work has shown that calpain, caspase-3, and the ubiquitin-proteasome pathway (UPP) are all activated in the diaphragm during prolonged MV. However, although it is established that both calpain and caspase-3 are important contributors to VIDD, the role that the UPP plays in the development of VIDD remains unknown. These experiments tested the hypothesis that inhibition of the UPP will protect the diaphragm against VIDD.
methodsThe authors tested this prediction in an established animal model of MV using a highly specific UPP inhibitor, epoxomicin, to prevent MV-induced activation of the proteasome in the diaphragm (n = 8 per group).
resultsThe results of this study reveal that inhibition of the UPP did not prevent ventilator-induced diaphragm muscle fiber atrophy and contractile dysfunction during 12 h of MV. Also, inhibition of the UPP does not affect MV-induced increases in calpain and caspase-3 activity in the diaphragm. Finally, administration of the proteasome inhibitor did not protect against the MV-induced increases in the expression of the E3 ligases, muscle ring finger-1 (MuRF1), and atrogin-1/MaFbx.
conclusionCollectively, these results indicate that proteasome activation does not play a required role in VIDD development during the first 12 h of MV.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.