Identification of Tf1 integration events in S. pombe under nonselective conditions.

Kristina E Cherry, Willis E Hearn, Osborne Y K Seshie, Teresa L Singleton

Abstract read

In one paragraph

Article in Gene, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

The Long Terminal Repeat Retrotransposons Tf1 and Tf2 of Schizosaccharomyces pombe.Microbiology spectrum · 2015
Review

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Kristina E CherryWinston Salem State University, 601 Martin Luther King Jr. Drive, WBA Science Building, Winston-Salem NC, USA. Electronic address: kriswilliams1234@gmail.com.

Willis E HearnWinston Salem State University, 601 Martin Luther King Jr. Drive, WBA Science Building, Winston-Salem NC, USA. Electronic address: willishearn19@gmail.com.

Osborne Y K SeshieWinston Salem State University, 601 Martin Luther King Jr. Drive, WBA Science Building, Winston-Salem NC, USA. Electronic address: oseshie111@rams.wssu.edu.

Teresa L SingletonWinston Salem State University, 601 Martin Luther King Jr. Drive, WBA Science Building, Winston-Salem NC, USA. Electronic address: singletont@wssu.edu.

Funding

RESEARCH COREP20MD002303 · NIMHD · WINSTON-SALEM STATE UNIVERSITY · PI FLACK, SYLVIA A · 2007 to 2011

$4.7M

NIMHD NIH HHS 1P20MD002303NIMHD NIH HHS P20 MD002303

6 · The paper itself

Abstract

Integration of retroviral elements into the host genome is a phenomena observed among many classes of retroviruses. Much information concerning the integration of retroviral elements has been documented based on in vitro analysis or expression of selectable markers. To identify possible Tf1 integration events within silent regions of the Schizosaccharomyces pombe genome, we focused on performing an in vivo genome-wide analysis of Tf1 integration events from the nonselective phase of the retrotransposition assay. We analyzed 1000 individual colonies streaked from four independent Tf1 transposed patches under nonselection conditions. Our analysis detected a population of G418(S)/neo(+) Tf1 integration events that would have been overlooked during the selective phase of the assay. Further RNA analysis from the G418(S)/neo(+) clones revealed 50% of clones expressing the neo selectable marker. Our data reveals Tf1's ability to insert within silent regions of S. pombe's genome.

Indexed as

Base SequenceDrug Resistance, FungalGene Expression Regulation, FungalGene SilencingGenome, FungalGentamicinsHydroxamic AcidsMolecular Sequence DataRetroelementsRNA, FungalSchizosaccharomycesSelection, Geneticantibiotic G 418GentamicinsHydroxamic AcidsRetroelementsRNA, Fungaltrichostatin AFission yeastHeterochromatinIntegrationRetrotransposonTrichostatin A

Identifiers

PMID24680781

DOI10.1016/j.gene.2014.03.030

PMCPMC4041700

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