ArticleBone2014
Bone marrow blood vessel ossification and "microvascular dead space" in rat and human long bone.
Article in Bone, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 27 citations in OpenAlex.
- Controlled Intramedullary Pressure Fluctuations Enhanced Periosteal and Endosteal Bone Formation in Young, but Not Old, Fischer-344 Rats.Biomimetics (Basel, Switzerland) · 2026Article
- Age- and sex-dependent bone marrow adiposity and distinct transcriptional trajectories in endothelial subtypes.Scientific reports · 2026Article
- Article
- Measuring tibial hemodynamics and metabolism at rest and after exercise using near-infrared spectroscopy.Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme · 2021Article
- Obstructive sleep apnea and fractures in children and adolescents.Journal of clinical sleep medicine : JCSM : official publication of the American Academy of Sleep Medicine · 2021Article
- Skeleton-vasculature chain reaction: a novel insight into the mystery of homeostasis.Bone research · 2021Review
- Low-Trauma Fracture Increases 12-Month Incidence of Cardiovascular Disease for Adults With Cerebral Palsy.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2020Article
- The Case for Measuring Long Bone Hemodynamics With Near-Infrared Spectroscopy.Frontiers in physiology · 2020Review
- A study of dynamic contrast-enhanced MR imaging features and influence factors of pelvic bone marrow in adult females.Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA · 2019Article
- Progressive ossification of the bone marrow vasculature with advancing age corresponds with reduced red blood cell count and percentage of circulating lymphocytes in male Fischer-344 rats.Microcirculation (New York, N.Y. : 1994) · 2019Article
- Discovery of a bone-like blood particle in the peripheral circulation of humans and rodents.Microcirculation (New York, N.Y. : 1994) · 2019Article
- Short-term intermittent PTH 1-34 administration and bone marrow blood vessel ossification in Mature and Middle-Aged C57BL/6 mice.Bone reports · 2019Article
- Intermittent PTH 1-34 administration improves the marrow microenvironment and endothelium-dependent vasodilation in bone arteries of aged rats.Journal of applied physiology (Bethesda, Md. : 1985) · 2018Article
- Effects of Aging on Fracture Healing.Current osteoporosis reports · 2017Review
- Mechanical, hormonal and metabolic influences on blood vessels, blood flow and bone.The Journal of endocrinology · 2017Review
- Imaging IGF-I uptake in growth plate cartilage using in vivo multiphoton microscopy.Journal of applied physiology (Bethesda, Md. : 1985) · 2017Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
Abstract
Severe calcification of the bone microvascular network was observed in rats, whereby the bone marrow blood vessels appeared ossified. This study sought to characterize the magnitude of ossification in relation to patent blood vessels and adipocyte content in femoral diaphyses. Additionally, this study confirmed the presence of ossified vessels in patients with arteriosclerotic vascular disease and peripheral vascular disease and cellulitis. Young (4-6 month; n=8) and old (22-24 month; n=8) male Fischer-344 rats were perfused with barium sulfate to visualize patent bone marrow blood vessels. Femoral shafts were processed for bone histomorphometry to quantify ossified (Goldner's Trichrome) and calcified (Alizarin Red) vessels. Adipocyte content was also determined. Additional femora (n=5/age group) were scanned via μCT to quantify microvascular ossification. Bone marrow blood vessels from the rats and the human patients were also isolated and examined via microscopy. Ossified vessels (rats and humans) had osteocyte lacunae on the vessel surfaces and "normal" vessels were transitioning into bone. The volume of ossified vessels was 4800% higher (p<0.05) in the old vs. young rats. Calcified and ossified vessel volumes per tissue volume and calcified vessel volume per patent vessel volume were augmented (p<0.05) 262%, 375% and 263%, respectively, in the old vs. young rats. Ossified and patent vessel number was higher (171%) and lower (40%), respectively, in the old vs. young rats. Finally, adipocyte volume per patent vessel volume was higher (86%) with age. This study is the first to report ossification of bone marrow blood vessels in rats and humans. Ossification presumably results in "microvascular dead space" in regard to loss of patency and vasomotor function as opposed to necrosis. Progression of bone microvascular ossification may provide the common link associated with age-related changes in bone and bone marrow. The clinical implications may be evident in the difficulties treating bone disease in the elderly.
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Registered trials
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