ArticleCytometry. Part A : the journal of the International Society for Analytical Cytology2014
Quantitative absorption cytometry for measuring red blood cell hemoglobin mass and volume.
Article in Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 45 citations in OpenAlex.
- Viscosity and density measurements on the cytosol of human red blood cells.Biophysical journal · 2025Article
- Chip-Based Optoacoustic Single-Cell Detection in Flow Using Point-Source Optimized Surface Acoustic Wave Transducers.ACS applied materials & interfaces · 2025Article
- Quantitative Measurements of Red Blood Cell Indices Using Spectroscopic Differential Phase-Contrast Microscopy.Chemical & biomedical imaging · 2023Article
- Dual-view transport of intensity phase imaging flow cytometry.Biomedical optics express · 2023Article
- Hemoglobin quantification in red blood cells via dry mass mapping based on UV absorption.Journal of biomedical optics · 2021Article
- A systems pharmacology model for gene therapy in sickle cell disease.CPT: pharmacometrics & systems pharmacology · 2021Article
- Quantitative absorption imaging of red blood cells to determine physical and mechanical properties.RSC advances · 2020Article
- High-throughput assessment of hemoglobin polymer in single red blood cells from sickle cell patients under controlled oxygen tension.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- A Microfluidic Deformability Assessment of Pathological Red Blood Cells Flowing in a Hyperbolic Converging Microchannel.Micromachines · 2019Article
- Optical force-induced nonlinearity and self-guiding of light in human red blood cell suspensions.Light, science & applications · 2019Article
- Single-shot dual-wavelength interferometric microscopy.Methods (San Diego, Calif.) · 2018Article
- Fluorescence Exclusion: A Simple Method to Assess Projected Surface, Volume and Morphology of Red Blood Cells Stored in Blood Bank.Frontiers in medicine · 2018Article
- Investigating cell functioning by theoretical analysis of cell-to-cell variability.European biophysics journal : EBJ · 2017Review
- Microfluidic microscopy-assisted label-free approach for cancer screening: automated microfluidic cytology for cancer screening.Medical & biological engineering & computing · 2017Review
- Phase and Index of Refraction Imaging by Hyperspectral Reflectance Confocal Microscopy.Molecules (Basel, Switzerland) · 2016Article
- A Microfluidic Approach for Inducing Cell Rotation by Means of Hydrodynamic Forces.Sensors (Basel, Switzerland) · 2016Article
- Single-cell measurement of red blood cell oxygen affinity.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Profiling individual human red blood cells using common-path diffraction optical tomography.Scientific reports · 2014Article
- In vivo volume and hemoglobin dynamics of human red blood cells.PLoS computational biology · 2014Article
- Biomechanical properties of red blood cells in health and disease towards microfluidics.Biomicrofluidics · 2014Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
We present an optical system, called the quantitative absorption cytometer (QAC), to measure the volume and hemoglobin mass of red blood cells flowing through a microfluidic channel. In contrast to clinical hematology analyzers, where cells are sphered in order for both volume and hemoglobin to be measured accurately, the QAC measures cells in their normal physiological shape. Human red blood cells are suspended in a refractive index-matching absorbing buffer, driven through a microfluidic channel, and imaged using a transmission light microscope onto a color camera. A red and a blue LED illuminate cells and images at each color are used to independently retrieve cell volume and hemoglobin mass. This system shows good agreement with red blood cell indices retrieved by a clinical hematology analyzer and in fact measures a smaller coefficient of variation of hemoglobin concentration. In addition to cell indices, the QAC returns height and mass maps of each measured cell. These quantitative images are valuable for analyzing the detailed morphology of individual cells as well as statistical outliers found in the data. We also measured red blood cells in hypertonic and hypotonic buffers to quantify the correlation between volume and hemoglobin mass under osmotic stress. Because this method is invariant to cell shape, even extremely nonspherical cells in hypertonic buffers can be measured accurately.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.