Evidence map›Paper›PMID 24677665›Full record

ArticleJournal of biomedical materials research. Part A2014

Evaluation of rhBMP-2 and bone marrow derived stromal cell mediated bone regeneration using transgenic fluorescent protein reporter mice.

Shalini V Gohil, Douglas J Adams, Peter Maye, David W Rowe, Lakshmi S Nair

Erratum issuedAbstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of biomedical materials research. Part A, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.5field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 16 citations in OpenAlex.

  1. Bone Tissue Engineering in the Treatment of Bone Defects.Pharmaceuticals (Basel, Switzerland) · 2022
    Review
  2. Article
  3. Article
  4. Article
  5. Tissue engineering. Part C, Methods · 2017
    Review
  6. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Shalini V GohilDepartment of Orthopedic Surgery, University of Connecticut Health Center, Farmington, Connecticut, 06032; Institute for Regenerative Engineering, The Raymond Beverly Sackler Center for Biomedical, Biological, Physical and Engineering Sciences, University of Connecticut Health Center, Farmington, Connecticut, 06032.
Douglas J Adams
Peter Maye
David W Rowe
Lakshmi S Nair
UConn Health · US

Funding

Skeletal Phenotyping of KOMP MiceR01AR063702 · NIAMS · UNIVERSITY OF CONNECTICUT SCH OF MED/DNT · PI ACKERT-BICKNELL, CHERYL LYNNE, ROWE, DAVID W. · 2013 to 2017
$3.2M
Targeted Correction of Dominant Mutations of Type I Collagen Causing Severe OIR01AR064381 · NIAMS · UNIVERSITY OF CONNECTICUT SCH OF MED/DNT · PI ROWE, DAVID W. · 2013 to 2017
$1.7M
NIAMS NIH HHS R01 AR063702NIAMS NIH HHS R01 AR064381
6 · The paper itself

Abstract

The aim of the study is use of transgenic fluorescent protein reporter mouse models to understand the cellular processes in recombinant human bone morphogenetic protein-2 (rhBMP-2) mediated bone formation. Bilateral parietal calvarial bone defects in Col3.6Topaz transgenic fluorescent osteoblast reporter mouse were used to understand the bone formation in the presence and absence of rhBMP2 and/or Col3.6Cyan bone marrow derived stromal cells (BMSCs), using collagen-hydroxyapatite matrix (Healos) as a biomaterial. The bone regeneration was not confined to the site of BMP-2 implantation and significant bone formation was observed in the neighboring defect site. Osteogenic cellular activity with overlying alizarin complexone staining was observed in both the defects indicating host cell induced mineralization. However, implantation of BMSCs along with rhBMP-2 demonstrated a donor cell derived bone formation. The presence of rhBMP-2 did not support host cell recruitment in the presence of donor cells. This study demonstrates the potential of multiple fluorescent reporters to understand the cellular processes involved in the bone regeneration process using biomaterials, growth factors, and/or stem cells.

Indexed as

Bone Marrow TransplantationBone RegenerationOsteogenesisAnimalsBone Marrow CellsBone Morphogenetic Protein 2Cells, ImmobilizedCHO CellsCollagenCricetinaeCricetulusDurapatiteGreen Fluorescent ProteinsHumansMiceMice, TransgenicBMP2 protein, humanBone Morphogenetic Protein 2CollagenCyan Fluorescent ProteinDurapatiteGreen Fluorescent ProteinsHealosRecombinant Proteinsbone morphogenetic proteinsbone regenerationcalvarial bone defect modelfluorescent protein reporter mousestem cells

Identifiers

PMID24677665
PMCPMC4894319
OpenAlexW1599494694

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.