ArticleRapid communications in mass spectrometry : RCM2014
Improved isobaric tandem mass tag quantification by ion mobility mass spectrometry.
Article in Rapid communications in mass spectrometry : RCM, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- In-depth patient-specific analysis of tumor heterogeneity in melanoma brain metastasis: Insights from spatial transcriptomics and multi-region bulk sequencing.Translational oncology · 2025Article
- Network proteomics of the Lewy body dementia brain reveals presynaptic signatures distinct from Alzheimer's disease.Molecular neurodegeneration · 2024Article
- Quantitative Peptidomics: General Considerations.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Oncogenic Proteomics Approaches for Translational Research and HIV-Associated Malignancy Mechanisms.Proteomes · 2023Review
- Chemical isotope labeling for quantitative proteomics.Mass spectrometry reviews · 2023Review
- Article
- Proteomics and Schizophrenia: The Evolution of a Great Partnership.Advances in experimental medicine and biology · 2022Article
- Metandem: An online software tool for mass spectrometry-based isobaric labeling metabolomics.Analytica chimica acta · 2019Article
- A New Quantification Method Using Electrochemical Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2019Article
- TomahaqCompanion: A Tool for the Creation and Analysis of Isobaric Label Based Multiplexed Targeted Assays.Journal of proteome research · 2019Article
- Sample Multiplexing Strategies in Quantitative Proteomics.Analytical chemistry · 2019Review
- New techniques, applications and perspectives in neuropeptide research.The Journal of experimental biology · 2018Review
- Recent advances in ion mobility-mass spectrometry for improved structural characterization of glycans and glycoconjugates.Current opinion in chemical biology · 2018Review
- Differential analysis of quantitative proteome and acetyl-proteome profiling between premenopausal and postmenopausal ovarian tissues.Clinical proteomics · 2018Article
- Mass Defect-Based N,N-Dimethyl Leucine Labels for Quantitative Proteomics and Amine Metabolomics of Pancreatic Cancer Cells.Analytical chemistry · 2017Article
- Simultaneous Proteomic Discovery and Targeted Monitoring using Liquid Chromatography, Ion Mobility Spectrometry, and Mass Spectrometry.Molecular & cellular proteomics : MCP · 2016Article
- Development and characterization of novel 8-plex DiLeu isobaric labels for quantitative proteomics and peptidomics.Rapid communications in mass spectrometry : RCM · 2015Article
- Sample multiplexing with cysteine-selective approaches: cysDML and cPILOT.Journal of the American Society for Mass Spectrometry · 2015Article
- Ion mobility-mass spectrometry strategies for untargeted systems, synthetic, and chemical biology.Current opinion in biotechnology · 2015Review
- Relative quantification of amine-containing metabolites using isobaric N,N-dimethyl leucine (DiLeu) reagents via LC-ESI-MS/MS and CE-ESI-MS/MS.The Analyst · 2015Article
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3 authors.
Funding
Abstract
rationaleIsobaric tandem mass tags are an attractive alternative to mass difference tags and label-free approaches for quantitative proteomics due to the high degree of multiplexing that can be performed with their implementation. A drawback of tandem mass tags are that the co-isolation and co-fragmentation of labeled peptide precursors can result in chimeric tandem mass (MS/MS) spectra that can underestimate the fold-change expression of each peptide. Ion mobility (IM) separations coupled to quadrupole time-of-flight (Q-TOF) instruments have the potential to mitigate MS/MS spectra chimeracy since IM-MS has the ability to separate ions based on charge, m/z, and collision cross section (CCS).
methodsTwo complex protein mixtures, labeled with DiLeu isobaric tandem mass tags in opposite ratios, were mixed together and analyzed by data-dependent LC/IM-MS/MS. The accuracy of reporters from interfering pairs was compared with and without IM separation.
resultsIM separation was able to mitigate isobaric interference from differentially charged interfering ion pairs, as well as pairs of the same charge. Of the eight example precursors shown, only one had reporters that remained compressed below the significance threshold after IM separation.
conclusionsThe results of this investigation demonstrate proof-of-principle that IM separation of tagged precursors prior to MS/MS fragmentation can help mitigate quantitative inaccuracies caused by isobaric interference. Future improvements of the method would include software for automated correction and use of higher resolution IM instrumentations.
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