ArticleRNA (New York, N.Y.)2014
miRNA sensitivity to Drosha levels correlates with pre-miRNA secondary structure.
Article in RNA (New York, N.Y.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 26 citations in OpenAlex.
- A Slow Dynamic RNA Switch Regulates Processing of microRNA-21.Journal of molecular biology · 2022Article
- Analysis of miRNA rare variants in amyotrophic lateral sclerosis andFrontiers in genetics · 2022Article
- Novel insight into miRNA biology and its role in the pathogenesis of systemic lupus erythematosus.Frontiers in immunology · 2022Review
- Steroidal Regulation of Oviductal microRNAs Is Associated with microRNA-Processing in Beef Cows.International journal of molecular sciences · 2021Article
- Extracellular vesicles as regulators of kidney function and disease.Intensive care medicine experimental · 2020Review
- Long noncoding RNA ZFAS1 promotes tumorigenesis and metastasis in nasopharyngeal carcinoma by sponging miR-892b to up-regulate LPAR1 expression.Journal of cellular and molecular medicine · 2020Article
- MicroRNAs: From Mechanism to Organism.Frontiers in cell and developmental biology · 2020Review
- Cross-Species Suppression of Hepatoma Cell Growth and Migration by a Schistosoma japonicum MicroRNA.Molecular therapy. Nucleic acids · 2019Article
- The relevance of microRNA in post-infarction left ventricular remodelling and heart failure.Heart failure reviews · 2019Review
- Post-transcriptional control of miRNA biogenesis.RNA (New York, N.Y.) · 2019Review
- Evaluation Expression of Microrna-93 and Integrin Β8 in Different Types of Glioma TumorsAsian Pacific journal of cancer prevention : APJCP · 2017Article
- The Drosophila Dicer-1 Partner Loquacious Enhances miRNA Processing from Hairpins with Unstable Structures at the Dicing Site.Cell reports · 2016Article
- Rbfox proteins regulate microRNA biogenesis by sequence-specific binding to their precursors and target downstream Dicer.Nucleic acids research · 2016Article
- The metabolome regulates the epigenetic landscape during naive-to-primed human embryonic stem cell transition.Nature cell biology · 2015Article
- Article
- Drosha inclusions are new components of dipeptide-repeat protein aggregates in FTLD-TDP and ALS C9orf72 expansion cases.Journal of neuropathology and experimental neurology · 2015Article
- Microprocessor mediates transcriptional termination of long noncoding RNA transcripts hosting microRNAs.Nature structural & molecular biology · 2015Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
microRNAs (miRNAs) are crucial for cellular development and homeostasis. In order to better understand regulation of miRNA biosynthesis, we studied cleavage of primary miRNAs by Drosha. While Drosha knockdown triggers an expected decrease of many mature miRNAs in human embryonic stem cells (hESC), a subset of miRNAs are not reduced. Statistical analysis of miRNA secondary structure and fold change of expression in response to Drosha knockdown showed that absence of mismatches in the central region of the hairpin, 5 and 9-12 nt from the Drosha cutting site conferred decreased sensitivity to Drosha knockdown. This suggests that, when limiting, Drosha processes miRNAs without mismatches more efficiently than mismatched miRNAs. This is important because Drosha expression changes over cellular development and the fold change of expression for miRNAs with mismatches in the central region correlates with Drosha levels. To examine the biochemical relationship directly, we overexpressed structural variants of miRNA-145, miRNA-137, miRNA-9, and miRNA-200b in HeLa cells with and without Drosha knockdown; for these miRNAs, elimination of mismatches in the central region increased, and addition of mismatches decreased their expression in an in vitro assay and in cells with low Drosha expression. Change in Drosha expression can be a biologically relevant mechanism by which eukaryotic cells control miRNA profiles. This phenomenon may explain the impact of point mutations outside the seed region of certain miRNAs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.