ArticleInvestigative ophthalmology & visual science2014
Cytokine deposition alters leukocyte morphology and initial recruitment of monocytes and γδT cells after corneal injury.
Article in Investigative ophthalmology & visual science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
12 citing papers in PubMed, 14 citations in OpenAlex.
- Macrophages increase in corneal epithelium and stroma after photorefractive keratectomy-like corneal abrasion and amplify ocular pain responses in rats.Experimental eye research · 2026Article
- Shared Phenotypes of Immune Cells Recruited to the Cornea and the Surface of the Lens in Response to Formation of Corneal Erosions.The American journal of pathology · 2025Article
- An Epithelial Abrasion Model for Studying Corneal Wound Healing.Journal of visualized experiments : JoVE · 2021Article
- Immune responses to injury and their links to eye disease.Translational research : the journal of laboratory and clinical medicine · 2021Review
- Macrophage recruitment in immune-privileged lens during capsule repair, necrotic fiber removal, and fibrosis.iScience · 2021Article
- An immune response to the avascular lens following wounding of the cornea involves ciliary zonule fibrils.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- Fibrosis: Shared Lessons From the Lens and Cornea.Anatomical record (Hoboken, N.J. : 2007) · 2020Review
- Axonal debris accumulates in corneal epithelial cells after intraepithelial corneal nerves are damaged: A focused Ion Beam Scanning Electron Microscopy (FIB-SEM) study.Experimental eye research · 2020Article
- Induction of Immune Surveillance of the Dysmorphogenic Lens.Scientific reports · 2017Article
- Review
- Comparison of the Anti-Inflammatory Effects of Artificial Tears in a Rat Model of Corneal Scraping.Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics · 2016Article
- Partial denervation of sub-basal axons persists following debridement wounds to the mouse cornea.Laboratory investigation; a journal of technical methods and pathology · 2015Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
purposeAn in vivo mouse model reproducibly induces recurrent epithelial erosions in wild-type mice spontaneously 2 weeks after a single 1.5-mm corneal debridement wound made with a dulled blade. When 1.5-mm wounds are made by a rotating burr so that the corneal epithelial basement membrane is removed, corneas heal without developing erosions. Here, we characterize differences in cytokine deposition and changes in leukocytes between 0 and 6 hours after dulled-blade and rotating-burr wounding.
methodsBALB/c mice were used to study 1.5-mm corneal wounds made using a dulled blade or a rotating burr. Mice were studied immediately after wounding (0 hour) and at 6 hours in vivo and in vitro in organ culture. Corneas, corneal extracts, and collagenase digests from naïve and wounded mice were used for three-dimensional (3D) confocal imaging, cytokine arrays, and flow cytometry.
resultsConfocal imaging showed CD45, a protein derived from leukocytes, accumulates at the wound edge by 3 and 6 hours after wounding in vivo but not in vitro with more CD45 accumulating after dulled-blade compared with rotating-burr wounds. Morphologic changes occurred in CD45+ leukocytes and higher levels for several cytokines were detected in the stromal wound bed within minutes following dulled-blade wounds. Flow cytometry showed significantly more monocytes (CD45+/CD11b+/Ly6C+) and γδT cells (CD45+/GL3+) recruited into the corneas of mice with dulled-blade wounds by 6 hours.
conclusionsDifferences in cytokine-driven leukocyte responses are seen after dulled-blade debridement compared with rotating-burr injury.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.