ArticlePlant physiology2014
Evolutionary convergence of cell-specific gene expression in independent lineages of C4 grasses.
Article in Plant physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
61 citing papers in PubMed, 1 synthesis or guideline pooled it, 121 citations in OpenAlex.
- Freeze-quenched maize mesophyll and bundle sheath separation uncovers bias in previous tissue-specific RNA-Seq data.Journal of experimental botany · 2017Pooled it
- Sulfur metabolism-dependent retrograde signaling for oxidative stress acclimation.Journal of experimental botany · 2026Review
- Exploring the diversity of the CO2-concentrating mechanism in different C4 subtypes.Journal of experimental botany · 2026Article
- Intercellular compartmentation of trehalose 6-phosphate metabolism in Setaria viridis leaves.Journal of experimental botany · 2025Article
- Phylogenetic diversity of light-dependent phosphorylation of Thr78 in Rubisco activase.Journal of experimental botany · 2025Article
- The metabolite transporters of C4 photosynthesis.The Plant cell · 2025Review
- Systems analysis of long-term heat stress responses in the C4 grass Setaria viridis.The Plant cell · 2025Article
- Regulation of Crassulacean acid metabolism at the protein level in Kalanchoë laxiflora.Plant physiology · 2025Article
- Genomic and Transcriptomic Insights into the Evolution of C4 Photosynthesis in Grasses.Genome biology and evolution · 2024Article
- Sugar sensing in C4 source leaves: a gap that needs to be filled.Journal of experimental botany · 2024Review
- GOLDEN2-like1 is sufficient but not necessary for chloroplast biogenesis in mesophyll cells of CThe Plant journal : for cell and molecular biology · 2024Article
- The pleiotropic functions of GOLDEN2-LIKE transcription factors in plants.Frontiers in plant science · 2024Review
- Compartmentation of photosynthesis gene expression in C4 maize depends on time of day.Plant physiology · 2023Article
- Photosynthesis and food security: the evolving story of CPhotosynthesis research · 2023Review
- Identification of Two Flip-Over Genes in Grass Family as Potential Signature of C4 Photosynthesis Evolution.International journal of molecular sciences · 2023Article
- Spatial expression patterns of genes encoding sugar sensors in leaves of C4 and C3 grasses.Annals of botany · 2023Article
- Review
- Computational Docking Reveals Co-Evolution of C4 Carbon Delivery Enzymes in Diverse Plants.International journal of molecular sciences · 2022Article
- Reduction of bundle sheath size boosts cyclic electron flow in CThe Plant journal : for cell and molecular biology · 2022Article
- Distinct CPlant direct · 2021Article
1 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Leaves of almost all C4 lineages separate the reactions of photosynthesis into the mesophyll (M) and bundle sheath (BS). The extent to which messenger RNA profiles of M and BS cells from independent C4 lineages resemble each other is not known. To address this, we conducted deep sequencing of RNA isolated from the M and BS of Setaria viridis and compared these data with publicly available information from maize (Zea mays). This revealed a high correlation (r=0.89) between the relative abundance of transcripts encoding proteins of the core C4 pathway in M and BS cells in these species, indicating significant convergence in transcript accumulation in these evolutionarily independent C4 lineages. We also found that the vast majority of genes encoding proteins of the C4 cycle in S. viridis are syntenic to homologs used by maize. In both lineages, 122 and 212 homologous transcription factors were preferentially expressed in the M and BS, respectively. Sixteen shared regulators of chloroplast biogenesis were identified, 14 of which were syntenic homologs in maize and S. viridis. In sorghum (Sorghum bicolor), a third C4 grass, we found that 82% of these trans-factors were also differentially expressed in either M or BS cells. Taken together, these data provide, to our knowledge, the first quantification of convergence in transcript abundance in the M and BS cells from independent lineages of C4 grasses. Furthermore, the repeated recruitment of syntenic homologs from large gene families strongly implies that parallel evolution of both structural genes and trans-factors underpins the polyphyletic evolution of this highly complex trait in the monocotyledons.
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Registered trials
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