ArticlePlant physiology2014
The heat shock factor A4A confers salt tolerance and is regulated by oxidative stress and the mitogen-activated protein kinases MPK3 and MPK6.
Article in Plant physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 112 papers, 1 of them a synthesis that pooled it.
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Who cites it
112 citing papers in PubMed, 1 synthesis or guideline pooled it, 249 citations in OpenAlex.
- Uncovering core regulators of multi-abiotic stress adaptation in Arabidopsis thaliana through integrative meta-analysis and machine learning with RT-qPCR validation.Scientific reports · 2026Pooled it
- Genome-wide analysis of the HSF gene family in lily and functional characterization of LdHSFC1 under heat stress.Plant cell reports · 2026Article
- MPK3-mediated phosphorylation inactivates MYB4 to promote FIT-dependent iron acquisition.EMBO reports · 2026Article
- Genome-Wide Analysis of the Hsf Family and Functional Characterization ofPlants (Basel, Switzerland) · 2026Article
- NPlants (Basel, Switzerland) · 2025Article
- Review
- Roles of ROS and NO in Plant Responses to Individual and Combined Salt Stress and Waterlogging.Antioxidants (Basel, Switzerland) · 2025Review
- Genome-wide identification of heat shock factors in Myricaria laxiflora and their response to abiotic stresses.BMC genomics · 2025Article
- Adapting Crops to Rising Temperatures: Understanding Heat Stress and Plant Resilience Mechanisms.International journal of molecular sciences · 2025Review
- OsLEML2 Regulates Rice Thermotolerance Through Auxin and ROS Homeostasis.Rice (New York, N.Y.) · 2025Article
- Article
- Article
- Plant Signaling Hormones and Transcription Factors: Key Regulators of Plant Responses to Growth, Development, and Stress.Plants (Basel, Switzerland) · 2025Review
- Phosphoproteomic Analysis of Maize Seedlings Provides Insights into the Mechanisms of Heat-Stress Tolerance.International journal of molecular sciences · 2025Article
- Comparative Transcriptome Analysis Reveals Mechanisms of Differential Salinity Tolerance BetweenGenes · 2024Article
- MPK4-mediated phosphorylation of PHYTOCHROME INTERACTING FACTOR4 controls thermosensing by regulating histone variant H2A.Z deposition.The Plant cell · 2024Article
- Heat-stress-responsive HvHSFA2e gene regulates the heat and drought tolerance in barley through modulation of phytohormone and secondary metabolic pathways.Plant cell reports · 2024Article
- Transcriptome Analysis of the Regulatory Mechanisms of Holly (Plants (Basel, Switzerland) · 2024Article
- Multiome in the Same Cell Reveals the Impact of Osmotic Stress on Arabidopsis Root Tip Development at Single-Cell Level.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Genome-Wide Analysis of theInternational journal of molecular sciences · 2024Article
52 more citing papers are in PubMed but not listed here.
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Authors and funding
14 authors at 4 institutions in 4 countries.
Funding
Abstract
Heat shock factors (HSFs) are principal regulators of plant responses to several abiotic stresses. Here, we show that estradiol-dependent induction of HSFA4A confers enhanced tolerance to salt and oxidative agents, whereas inactivation of HSFA4A results in hypersensitivity to salt stress in Arabidopsis (Arabidopsis thaliana). Estradiol induction of HSFA4A in transgenic plants decreases, while the knockout hsfa4a mutation elevates hydrogen peroxide accumulation and lipid peroxidation. Overexpression of HSFA4A alters the transcription of a large set of genes regulated by oxidative stress. In yeast (Saccharomyces cerevisiae) two-hybrid and bimolecular fluorescence complementation assays, HSFA4A shows homomeric interaction, which is reduced by alanine replacement of three conserved cysteine residues. HSFA4A interacts with mitogen-activated protein kinases MPK3 and MPK6 in yeast and plant cells. MPK3 and MPK6 phosphorylate HSFA4A in vitro on three distinct sites, serine-309 being the major phosphorylation site. Activation of the MPK3 and MPK6 mitogen-activated protein kinase pathway led to the transcriptional activation of the HEAT SHOCK PROTEIN17.6A gene. In agreement that mutation of serine-309 to alanine strongly diminished phosphorylation of HSFA4A, it also strongly reduced the transcriptional activation of HEAT SHOCK PROTEIN17.6A. These data suggest that HSFA4A is a substrate of the MPK3/MPK6 signaling and that it regulates stress responses in Arabidopsis.
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